Multiphoton microscopy at an excitation wavelength of 1.26 μm

Karsten Plamann; Gilbert Boyer; Denis Chemla

doi:10.1117/12.500487

Multiphoton microscopy at an excitation wavelength of 1.26 μm

Karsten Plamann
, Gilbert Boyer
, Denis Chemla

Research output: Contribution to journal › Conference article › peer-review

Abstract

We present a multiphoton microscope using a Cr⁴⁺:forsterite femtosecond laser with an emission wavelength of 1260 nm for the excitation of the multiphoton processes. This wavelength is well adapted to the "optical window" in biological tissues and permits to reach higher imaging depths than systems using more conventional titanium:sapphire laser sources. The paper describes the experimental set-up and reports on first results on human cornea and skin samples.

Original language	English
Pages (from-to)	42-50
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5139
DOIs	https://doi.org/10.1117/12.500487
Publication status	Published - 1 Jan 2003
Event	Confocal, Multiphoton, and Nonlinear Microscopic Imaging - Munich, Germany Duration: 22 Jun 2003 → 23 Jun 2003

Keywords

Cr:forsterite laser
Dermatology
Imaging depth
Multi-photon microscopy
Optical window in tissue
Refractive surgery

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10.1117/12.500487

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title = "Multiphoton microscopy at an excitation wavelength of 1.26 μm",

abstract = "We present a multiphoton microscope using a Cr4+:forsterite femtosecond laser with an emission wavelength of 1260 nm for the excitation of the multiphoton processes. This wavelength is well adapted to the {"}optical window{"} in biological tissues and permits to reach higher imaging depths than systems using more conventional titanium:sapphire laser sources. The paper describes the experimental set-up and reports on first results on human cornea and skin samples.",

keywords = "Cr:forsterite laser, Dermatology, Imaging depth, Multi-photon microscopy, Optical window in tissue, Refractive surgery",

author = "Karsten Plamann and Gilbert Boyer and Denis Chemla",

year = "2003",

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doi = "10.1117/12.500487",

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journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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TY - JOUR

T1 - Multiphoton microscopy at an excitation wavelength of 1.26 μm

AU - Plamann, Karsten

AU - Boyer, Gilbert

AU - Chemla, Denis

PY - 2003/1/1

Y1 - 2003/1/1

N2 - We present a multiphoton microscope using a Cr4+:forsterite femtosecond laser with an emission wavelength of 1260 nm for the excitation of the multiphoton processes. This wavelength is well adapted to the "optical window" in biological tissues and permits to reach higher imaging depths than systems using more conventional titanium:sapphire laser sources. The paper describes the experimental set-up and reports on first results on human cornea and skin samples.

AB - We present a multiphoton microscope using a Cr4+:forsterite femtosecond laser with an emission wavelength of 1260 nm for the excitation of the multiphoton processes. This wavelength is well adapted to the "optical window" in biological tissues and permits to reach higher imaging depths than systems using more conventional titanium:sapphire laser sources. The paper describes the experimental set-up and reports on first results on human cornea and skin samples.

KW - Cr:forsterite laser

KW - Dermatology

KW - Imaging depth

KW - Multi-photon microscopy

KW - Optical window in tissue

KW - Refractive surgery

U2 - 10.1117/12.500487

DO - 10.1117/12.500487

M3 - Conference article

AN - SCOPUS:1342310559

SN - 0277-786X

VL - 5139

SP - 42

EP - 50

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Confocal, Multiphoton, and Nonlinear Microscopic Imaging

Y2 - 22 June 2003 through 23 June 2003

ER -

Multiphoton microscopy at an excitation wavelength of 1.26 μm

Abstract

Keywords

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