TY - JOUR
T1 - Nonlinear optical imaging of lyotropic cholesteric liquid crystals
AU - Deniset-Besseau, Ariane
AU - Peixoto, Paulo De Sa
AU - Gervaise Mosser, Mosser
AU - Schanne-Klein, Marie Claire
PY - 2010/1/18
Y1 - 2010/1/18
N2 - We use nonlinear optical microscopy combining Second Harmonic Generation (SHG) microscopy and Two-Photon Excited Fluorescence (2PEF) signals to characterize collagen lyotropic liquid crystals. We show that SHG signals provide highly contrasted images of the three-dimensional texture of cholesteric patterns with submicrometer lateral resolution. Moreover, simultaneous recording of the 2PEF signal enables in situ quantitative mapping of the molecular concentration and its correlation with the observed textures. We apply this technique to the characterization of biomimetic textures obtained in concentrated collagen liquid solutions. We successfully image biologically relevant organizations that are similar to the collagen organization found as a stabilized state in compact bones.
AB - We use nonlinear optical microscopy combining Second Harmonic Generation (SHG) microscopy and Two-Photon Excited Fluorescence (2PEF) signals to characterize collagen lyotropic liquid crystals. We show that SHG signals provide highly contrasted images of the three-dimensional texture of cholesteric patterns with submicrometer lateral resolution. Moreover, simultaneous recording of the 2PEF signal enables in situ quantitative mapping of the molecular concentration and its correlation with the observed textures. We apply this technique to the characterization of biomimetic textures obtained in concentrated collagen liquid solutions. We successfully image biologically relevant organizations that are similar to the collagen organization found as a stabilized state in compact bones.
U2 - 10.1364/OE.18.001113
DO - 10.1364/OE.18.001113
M3 - Article
C2 - 20173934
AN - SCOPUS:75249095617
SN - 1094-4087
VL - 18
SP - 1113
EP - 1121
JO - Optics Express
JF - Optics Express
IS - 2
ER -