Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction

Myriam Lazard; Marc Mirande; Jean Pierre Waller

doi:10.1016/0014-5793(87)80750-0

Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction

Myriam Lazard
, Marc Mirande
, Jean Pierre Waller

CNRS-UPR U. Propre de Recherche 9063

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The effect of phenylalanine restriction on the level of expression of phenylalanyl-tRNA synthetase from cultured Chinese hamster ovary cells was investigated. By lowering the phenylalanine concentration from 200 to 2 μM, cell growth was arrested, tRNA^Phe aminoacylation level was rapidly and specifically decreased and phenylalanyl-tRNA synthetase was derepressed. The progressive 2-fold elevation of phenylalanyl- tRNA synthetase level was determined by activity measurement and immunotitration. None of the other aminoacyl-tRNA synthetases tested were significantly affected.

Original language	English
Pages (from-to)	27-30
Number of pages	4
Journal	FEBS Letters
Volume	216
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80750-0
Publication status	Published - 25 May 1987
Externally published	Yes

Keywords

(CHO cell)
Phenylalanyl-tRNA synthetase
Physiological regulation

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10.1016/0014-5793(87)80750-0

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title = "Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction",

abstract = "The effect of phenylalanine restriction on the level of expression of phenylalanyl-tRNA synthetase from cultured Chinese hamster ovary cells was investigated. By lowering the phenylalanine concentration from 200 to 2 μM, cell growth was arrested, tRNAPhe aminoacylation level was rapidly and specifically decreased and phenylalanyl-tRNA synthetase was derepressed. The progressive 2-fold elevation of phenylalanyl- tRNA synthetase level was determined by activity measurement and immunotitration. None of the other aminoacyl-tRNA synthetases tested were significantly affected.",

keywords = "(CHO cell), Phenylalanyl-tRNA synthetase, Physiological regulation",

author = "Myriam Lazard and Marc Mirande and Waller, \{Jean Pierre\}",

year = "1987",

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doi = "10.1016/0014-5793(87)80750-0",

language = "English",

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T1 - Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction

AU - Lazard, Myriam

AU - Mirande, Marc

AU - Waller, Jean Pierre

PY - 1987/5/25

Y1 - 1987/5/25

N2 - The effect of phenylalanine restriction on the level of expression of phenylalanyl-tRNA synthetase from cultured Chinese hamster ovary cells was investigated. By lowering the phenylalanine concentration from 200 to 2 μM, cell growth was arrested, tRNAPhe aminoacylation level was rapidly and specifically decreased and phenylalanyl-tRNA synthetase was derepressed. The progressive 2-fold elevation of phenylalanyl- tRNA synthetase level was determined by activity measurement and immunotitration. None of the other aminoacyl-tRNA synthetases tested were significantly affected.

AB - The effect of phenylalanine restriction on the level of expression of phenylalanyl-tRNA synthetase from cultured Chinese hamster ovary cells was investigated. By lowering the phenylalanine concentration from 200 to 2 μM, cell growth was arrested, tRNAPhe aminoacylation level was rapidly and specifically decreased and phenylalanyl-tRNA synthetase was derepressed. The progressive 2-fold elevation of phenylalanyl- tRNA synthetase level was determined by activity measurement and immunotitration. None of the other aminoacyl-tRNA synthetases tested were significantly affected.

KW - (CHO cell)

KW - Phenylalanyl-tRNA synthetase

KW - Physiological regulation

U2 - 10.1016/0014-5793(87)80750-0

DO - 10.1016/0014-5793(87)80750-0

M3 - Article

C2 - 3582665

AN - SCOPUS:0023664434

SN - 0014-5793

VL - 216

SP - 27

EP - 30

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction

Abstract

Keywords

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