Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging

Paolo Matteini; Fulvio Ratto; Francesca Rossi; Riccardo Cicchi; Chiara Stringari; Dimitrios Kapsokalyvas; Francesco S. Pavone; Roberto Pini

doi:10.1364/OE.17.004868

Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging

Paolo Matteini
, Fulvio Ratto
, Francesca Rossi
, Riccardo Cicchi
, Chiara Stringari
, Dimitrios Kapsokalyvas
, Francesco S. Pavone
, Roberto Pini

Research output: Contribution to journal › Article › peer-review

Abstract

The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues.

Original language	English
Pages (from-to)	4868-4878
Number of pages	11
Journal	Optics Express
Volume	17
Issue number	6
DOIs	https://doi.org/10.1364/OE.17.004868
Publication status	Published - 16 Mar 2009
Externally published	Yes

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title = "Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging",

abstract = "The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues.",

author = "Paolo Matteini and Fulvio Ratto and Francesca Rossi and Riccardo Cicchi and Chiara Stringari and Dimitrios Kapsokalyvas and Pavone, \{Francesco S.\} and Roberto Pini",

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AU - Matteini, Paolo

AU - Ratto, Fulvio

AU - Rossi, Francesca

AU - Cicchi, Riccardo

AU - Stringari, Chiara

AU - Kapsokalyvas, Dimitrios

AU - Pavone, Francesco S.

AU - Pini, Roberto

PY - 2009/3/16

Y1 - 2009/3/16

N2 - The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues.

AB - The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues.

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DO - 10.1364/OE.17.004868

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SN - 1094-4087

VL - 17

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EP - 4878

JO - Optics Express

JF - Optics Express

IS - 6

ER -

Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging

Abstract

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