Polarization-resolved SHG microscopy of rat-tail tendon with controlled mechanical strain

I. Gusachenko, Y. Goulam Houssen, V. Tran, J. M. Allain, M. C. Schanne-Klein

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We combined polarization-resolved SHG microscopy with mechanical assays in rat-tail-tendon and measured collagen remodeling upon controlled stretching. This approach aimed to analyze the relationship between macroscopic response and sub-micrometer scale organization of collagen fibrils. We observed a straightening of the crimps followed by a sliding of the fibrils with increasing stretching of the tendon fascicles. Polarization resolution of the SHG images provided complementary information about the orientation dispersion of collagen fibrils within the focal volume and enabled monitoring of collagen remodeling at the sub-micrometer scale. Our approach can be readily generalized to other tissues and should bring new valuable information about biomechanics of microstructured tissues.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2013
PublisherOptical Society of America
ISBN (Print)9780819496461
Publication statusPublished - 1 Jan 2013
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2013 - Munich, Germany
Duration: 12 May 201316 May 2013

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2013
Country/TerritoryGermany
CityMunich
Period12/05/1316/05/13

Keywords

  • Biological tissues
  • Biomechanics
  • Collagen
  • Multiphoton microscopy
  • Polarimetry
  • Second harmonic generation

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