Pores formation on cell membranes by hederacolchiside a1 leads to a rapid release of proteins for cytosolic subproteome analysis

Gabriel D. Mazzucchelli; Nicolas A. Cellier; Vakhtang Mshviladzade; Riad Elias; Yong Ho Shim; David Touboul; Loïc Quinton; Alain Brunelle; Olivier Laprévote; Edwin A. De Pauw; Marie Claire A. De Pauw-Gillet

doi:10.1021/pr7006973

Pores formation on cell membranes by hederacolchiside a1 leads to a rapid release of proteins for cytosolic subproteome analysis

Gabriel D. Mazzucchelli
, Nicolas A. Cellier
, Vakhtang Mshviladzade
, Riad Elias
, Yong Ho Shim
, David Touboul
, Loïc Quinton
, Alain Brunelle
, Olivier Laprévote
, Edwin A. De Pauw
, Marie Claire A. De Pauw-Gillet

Research output: Contribution to journal › Article › peer-review

Abstract

Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.

Original language	English
Pages (from-to)	1683-1692
Number of pages	10
Journal	Journal of Proteome Research
Volume	7
Issue number	4
DOIs	https://doi.org/10.1021/pr7006973
Publication status	Published - 1 Apr 2008
Externally published	Yes

Keywords

2D-LC-MS/M
CMC
Fractionation
Mel-5, 2D gels
Pores
Saponin
Soluble biomarkers
TOF-SIMS

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10.1021/pr7006973

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Mazzucchelli, G. D., Cellier, N. A., Mshviladzade, V., Elias, R., Shim, Y. H., Touboul, D., Quinton, L., Brunelle, A., Laprévote, O., De Pauw, E. A., & De Pauw-Gillet, M. C. A. (2008). Pores formation on cell membranes by hederacolchiside a1 leads to a rapid release of proteins for cytosolic subproteome analysis. Journal of Proteome Research, 7(4), 1683-1692. https://doi.org/10.1021/pr7006973

@article{ae969c9090c94f77b52967df650b398d,

title = "Pores formation on cell membranes by hederacolchiside a1 leads to a rapid release of proteins for cytosolic subproteome analysis",

abstract = "Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.",

keywords = "2D-LC-MS/M, CMC, Fractionation, Mel-5, 2D gels, Pores, Saponin, Soluble biomarkers, TOF-SIMS",

author = "Mazzucchelli, \{Gabriel D.\} and Cellier, \{Nicolas A.\} and Vakhtang Mshviladzade and Riad Elias and Shim, \{Yong Ho\} and David Touboul and Lo{\"i}c Quinton and Alain Brunelle and Olivier Lapr{\'e}vote and \{De Pauw\}, \{Edwin A.\} and \{De Pauw-Gillet\}, \{Marie Claire A.\}",

year = "2008",

month = apr,

day = "1",

doi = "10.1021/pr7006973",

language = "English",

volume = "7",

pages = "1683--1692",

journal = "Journal of Proteome Research",

issn = "1535-3893",

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Mazzucchelli, GD, Cellier, NA, Mshviladzade, V, Elias, R, Shim, YH, Touboul, D, Quinton, L, Brunelle, A, Laprévote, O, De Pauw, EA & De Pauw-Gillet, MCA 2008, 'Pores formation on cell membranes by hederacolchiside a1 leads to a rapid release of proteins for cytosolic subproteome analysis', Journal of Proteome Research, vol. 7, no. 4, pp. 1683-1692. https://doi.org/10.1021/pr7006973

TY - JOUR

T1 - Pores formation on cell membranes by hederacolchiside a1 leads to a rapid release of proteins for cytosolic subproteome analysis

AU - Mazzucchelli, Gabriel D.

AU - Cellier, Nicolas A.

AU - Mshviladzade, Vakhtang

AU - Elias, Riad

AU - Shim, Yong Ho

AU - Touboul, David

AU - Quinton, Loïc

AU - Brunelle, Alain

AU - Laprévote, Olivier

AU - De Pauw, Edwin A.

AU - De Pauw-Gillet, Marie Claire A.

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.

AB - Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.

KW - 2D-LC-MS/M

KW - CMC

KW - Fractionation

KW - Mel-5, 2D gels

KW - Pores

KW - Saponin

KW - Soluble biomarkers

KW - TOF-SIMS

U2 - 10.1021/pr7006973

DO - 10.1021/pr7006973

M3 - Article

C2 - 18338859

AN - SCOPUS:45549104698

SN - 1535-3893

VL - 7

SP - 1683

EP - 1692

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 4

ER -

Pores formation on cell membranes by hederacolchiside a1 leads to a rapid release of proteins for cytosolic subproteome analysis

Abstract

Keywords

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