Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

K. W. Plaxco; A. M. Mathiowetz; W. A. Goddard

doi:10.1073/pnas.86.24.9841

Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

K. W. Plaxco, A. M. Mathiowetz, W. A. Goddard

California Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

Original language	English
Pages (from-to)	9841-9845
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Issue number	24
DOIs	https://doi.org/10.1073/pnas.86.24.9841
Publication status	Published - 1 Dec 1989
Externally published	Yes

Keywords

Protein-DNA interactions
Salmonella typhimurium
helix-turn-helix motif
molecular modeling
structure-function relationships

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10.1073/pnas.86.24.9841

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abstract = "Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.",

keywords = "Protein-DNA interactions, Salmonella typhimurium, helix-turn-helix motif, molecular modeling, structure-function relationships",

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AU - Goddard, W. A.

PY - 1989/12/1

Y1 - 1989/12/1

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AB - Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

KW - Protein-DNA interactions

KW - Salmonella typhimurium

KW - helix-turn-helix motif

KW - molecular modeling

KW - structure-function relationships

U2 - 10.1073/pnas.86.24.9841

DO - 10.1073/pnas.86.24.9841

M3 - Article

C2 - 2602378

AN - SCOPUS:0024787666

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VL - 86

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

Abstract

Keywords

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