Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

K. W. Plaxco; A. M. Mathiowetz; W. A. Goddard

doi:10.1073/pnas.86.24.9841

Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

K. W. Plaxco
, A. M. Mathiowetz
, W. A. Goddard

California Institute of Technology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

Original language	English
Pages (from-to)	9841-9845
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Issue number	24
DOIs	https://doi.org/10.1073/pnas.86.24.9841
Publication status	Published - 1 Dec 1989
Externally published	Yes

Keywords

Protein-DNA interactions
Salmonella typhimurium
helix-turn-helix motif
molecular modeling
structure-function relationships

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10.1073/pnas.86.24.9841

Cite this

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abstract = "Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.",

keywords = "Protein-DNA interactions, Salmonella typhimurium, helix-turn-helix motif, molecular modeling, structure-function relationships",

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AU - Plaxco, K. W.

AU - Mathiowetz, A. M.

AU - Goddard, W. A.

PY - 1989/12/1

Y1 - 1989/12/1

N2 - Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

AB - Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

KW - Protein-DNA interactions

KW - Salmonella typhimurium

KW - helix-turn-helix motif

KW - molecular modeling

KW - structure-function relationships

U2 - 10.1073/pnas.86.24.9841

DO - 10.1073/pnas.86.24.9841

M3 - Article

C2 - 2602378

AN - SCOPUS:0024787666

SN - 0027-8424

VL - 86

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

Abstract

Keywords

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