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Probing living cells by terahertz attenuated total reflection. Application to permeabilization dynamics

  • G. Gallot
  • , X. Zheng
  • , M. Grognot
  • , A. Azan
  • , T. Garcia-Sanchez
  • , L. Descamps
  • , L. M. Mir
  • Institut Polytechnique de Paris
  • Centre national de la recherche scientifique

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Using attenuated total reflection (ATR) in the terahertz domain [1], we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live epithelial cells. We show that terahertz modalities are more sensitive than fluorescence microscopy which is the reference optical technique for electropermeabilization. We also propose analytical models for the influx and efflux of non-permeant molecules through permeabilized cell membranes by electropermeabilization or by detergent.

Original languageEnglish
Title of host publication2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728104690
DOIs
Publication statusPublished - 1 Jun 2019
Event2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019 - Munich, Germany
Duration: 23 Jun 201927 Jun 2019

Publication series

Name2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019

Conference

Conference2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
Country/TerritoryGermany
CityMunich
Period23/06/1927/06/19

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