@inbook{192aefc52287409f8bce9bd19802ec61,
title = "Protection-Based Assays to Measure Aminoacyl-tRNA Binding to Translation Initiation Factors",
abstract = "To decipher the mechanisms of translation initiation, the stability of the complexes between tRNA and initiation factors has to be evaluated in a routine manner. A convenient method to measure the parameters of binding of an aminoacyl-tRNA to an initiation factor results from the property that, when specifically complexed to a protein, the aminoacyl-tRNA often resists spontaneous deacylation. This chapter describes the preparation of suitable aminoacyl-tRNA ligands and their use in evaluating the stability of their complexes with various initiation factors, such as e/aIF2 and e/aIF5B. The advantages and the limitations of the method are discussed.",
author = "Yves Mechulam and Laurent Guillon and Laure Yatime and Sylvain Blanquet and Emmanuelle Schmitt",
year = "2007",
month = jan,
day = "1",
doi = "10.1016/S0076-6879(07)30011-6",
language = "English",
isbn = "9780123739698",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "265--281",
booktitle = "Translation Initiation",
}