Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

Marianne Grognot; Guilhem Gallot

doi:10.1063/1.4930168

Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

Marianne Grognot
, Guilhem Gallot

Institut Polytechnique de Paris

Research output: Contribution to journal › Article › peer-review

Abstract

Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.

Original language	English
Article number	103702
Journal	Applied Physics Letters
Volume	107
Issue number	10
DOIs	https://doi.org/10.1063/1.4930168
Publication status	Published - 7 Sept 2015

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title = "Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection",

abstract = "Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.",

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AB - Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.

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VL - 107

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Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

Abstract

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