TY - JOUR
T1 - Rapid and sensitive identification of uropathogenic Escherichia coli using a surface-enhanced-Raman-scattering-based biochip
AU - Andrei, Cristina Cassiana
AU - Moraillon, Anne
AU - Lau, Stephanie
AU - Felidj, Nordin
AU - Yamakawa, Nao
AU - Bouckaert, Julie
AU - Larquet, Eric
AU - Boukherroub, Rabah
AU - Ozanam, François
AU - Szunerits, Sabine
AU - Chantal Gouget-Laemmel, Anne
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Rapid, selective and sensitive sensing of bacteria remains challenging. We report on a highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS)-based sensing approach for the detection of uropathogenic Escherichia coli (E. coli) bacteria in urine. The assay is based on the specific capture of the bacteria followed by interaction with cetyltrimethylammonium bromide (CTAB)-stabilised gold nanorods (Au NRS) as SERS markers. High sensitivity up to 10 CFU mL-1 is achieved by optimizing the capture interface based on hydrogenated amorphous silicon a-Si:H thin films. The integration of CH3O-PEG750 onto a-Si:H gives the sensing interface an efficient anti-fouling character, while covalent linkage of antibodies directed against the major type-1 fimbrial pilin FimA of the human pathogen E. coli results in the specific trapping of fimbriated E. coli onto the SERS substrate and their spectral fingerprint identification.
AB - Rapid, selective and sensitive sensing of bacteria remains challenging. We report on a highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS)-based sensing approach for the detection of uropathogenic Escherichia coli (E. coli) bacteria in urine. The assay is based on the specific capture of the bacteria followed by interaction with cetyltrimethylammonium bromide (CTAB)-stabilised gold nanorods (Au NRS) as SERS markers. High sensitivity up to 10 CFU mL-1 is achieved by optimizing the capture interface based on hydrogenated amorphous silicon a-Si:H thin films. The integration of CH3O-PEG750 onto a-Si:H gives the sensing interface an efficient anti-fouling character, while covalent linkage of antibodies directed against the major type-1 fimbrial pilin FimA of the human pathogen E. coli results in the specific trapping of fimbriated E. coli onto the SERS substrate and their spectral fingerprint identification.
KW - Biochips
KW - Escherichia coli
KW - Gold nanorods (Au NRs)
KW - Hydrogenated amorphous silicon (a-Si:H)
KW - Principle component analysis (PCA)
KW - Surface-enhanced Raman spectroscopy (SERS)
U2 - 10.1016/j.talanta.2020.121174
DO - 10.1016/j.talanta.2020.121174
M3 - Article
C2 - 32887096
AN - SCOPUS:85085711719
SN - 0039-9140
VL - 219
JO - Talanta
JF - Talanta
M1 - 121174
ER -