Red blood cell sickling in microfluidic droplets

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We have developed a droplet-based microfluidic device to study the red blood cell sickling process associated with sickle cell anaemia. A primary trigger of red blood cell sickling is low oxygen partial pressure. By controlling the oxygen partial pressure of the incoming oil and aqueous phase, a microdroplet is subjected to a rapid change in oxygen concentration. Using a fluorescent oxygen probe, the oxygen change was shown to occur in less than a second and concentrated during and directly after droplet formation. The deoxygenation of droplets is shown to trigger red blood cell sickling in microdroplets.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages493-495
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 1 Jan 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Microdroplets
  • Oxygen
  • Red blood cells
  • Sickle cell anaemia
  • Single cell

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