Removing artifacts in Second Harmonic Generation imaging by interferometry

Maxime Pinsard; Margaux Schmeltz; Jarno N. Van Der Kolk; Kessen Patten; Lora Ramunno; Marie Claire Schanne-Klein; François Légaré

doi:10.1109/PN.2019.8819578

Removing artifacts in Second Harmonic Generation imaging by interferometry

Maxime Pinsard
, Margaux Schmeltz
, Jarno N. Van Der Kolk
, Kessen Patten
, Lora Ramunno
, Marie Claire Schanne-Klein
, François Légaré

INRS-ÉMT
Institut Polytechnique de Paris
Department of Physics
University of Ottawa
Centre Armand-Frappier Santé Biotechnologie

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

— We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.

Original language	English
Title of host publication	2019 Photonics North, PN 2019
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781728137384
DOIs	https://doi.org/10.1109/PN.2019.8819578
Publication status	Published - 1 May 2019
Event	2019 Photonics North, PN 2019 - Quebec City, Canada Duration: 21 May 2019 → 23 May 2019

Publication series

Name	2019 Photonics North, PN 2019

Conference

Conference	2019 Photonics North, PN 2019
Country/Territory	Canada
City	Quebec City
Period	21/05/19 → 23/05/19

Keywords

Interferometry
Multiphoton microscopy
imaging artifacts

Access to Document

10.1109/PN.2019.8819578

Cite this

Pinsard, M., Schmeltz, M., Van Der Kolk, J. N., Patten, K., Ramunno, L., Schanne-Klein, M. C., & Légaré, F. (2019). Removing artifacts in Second Harmonic Generation imaging by interferometry. In 2019 Photonics North, PN 2019 Article 8819578 (2019 Photonics North, PN 2019). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/PN.2019.8819578

@inproceedings{8068d612975f44b4aafc6afdf5f482ed,

title = "Removing artifacts in Second Harmonic Generation imaging by interferometry",

abstract = "{\textemdash} We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.",

keywords = "Interferometry, Multiphoton microscopy, imaging artifacts",

author = "Maxime Pinsard and Margaux Schmeltz and \{Van Der Kolk\}, \{Jarno N.\} and Kessen Patten and Lora Ramunno and Schanne-Klein, \{Marie Claire\} and Fran{\c c}ois L{\'e}gar{\'e}",

note = "Publisher Copyright: {\textcopyright} 2019 IEEE.; 2019 Photonics North, PN 2019 ; Conference date: 21-05-2019 Through 23-05-2019",

year = "2019",

month = may,

day = "1",

doi = "10.1109/PN.2019.8819578",

language = "English",

series = "2019 Photonics North, PN 2019",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

booktitle = "2019 Photonics North, PN 2019",

}

Pinsard, M, Schmeltz, M, Van Der Kolk, JN, Patten, K, Ramunno, L, Schanne-Klein, MC & Légaré, F 2019, Removing artifacts in Second Harmonic Generation imaging by interferometry. in 2019 Photonics North, PN 2019., 8819578, 2019 Photonics North, PN 2019, Institute of Electrical and Electronics Engineers Inc., 2019 Photonics North, PN 2019, Quebec City, Canada, 21/05/19. https://doi.org/10.1109/PN.2019.8819578

Removing artifacts in Second Harmonic Generation imaging by interferometry. / Pinsard, Maxime; Schmeltz, Margaux; Van Der Kolk, Jarno N. et al.
2019 Photonics North, PN 2019. Institute of Electrical and Electronics Engineers Inc., 2019. 8819578 (2019 Photonics North, PN 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Removing artifacts in Second Harmonic Generation imaging by interferometry

AU - Pinsard, Maxime

AU - Schmeltz, Margaux

AU - Van Der Kolk, Jarno N.

AU - Patten, Kessen

AU - Ramunno, Lora

AU - Schanne-Klein, Marie Claire

AU - Légaré, François

PY - 2019/5/1

Y1 - 2019/5/1

N2 - — We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.

AB - — We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.

KW - Interferometry

KW - Multiphoton microscopy

KW - imaging artifacts

U2 - 10.1109/PN.2019.8819578

DO - 10.1109/PN.2019.8819578

M3 - Conference contribution

AN - SCOPUS:85072763106

T3 - 2019 Photonics North, PN 2019

BT - 2019 Photonics North, PN 2019

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2019 Photonics North, PN 2019

Y2 - 21 May 2019 through 23 May 2019

ER -

Removing artifacts in Second Harmonic Generation imaging by interferometry

Abstract

Publication series

Conference

Keywords

Access to Document

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Cite this