RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling

Cyrille Jeancolas; Yoshiya J. Matsubara; Mykhailo Vybornyi; Camille N. Lambert; Alex Blokhuis; Thomas Alline; Andrew D. Griffiths; Sandeep Ameta; Sandeep Krishna; Philippe Nghe

doi:10.1039/d1cc02290c

RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling

Cyrille Jeancolas
, Yoshiya J. Matsubara
, Mykhailo Vybornyi
, Camille N. Lambert
, Alex Blokhuis
, Thomas Alline
, Andrew D. Griffiths
, Sandeep Ameta
, Sandeep Krishna
, Philippe Nghe

École Polytechnique

Centre national de la recherche scientifique
Collège de France
National Centre for Biological Sciences
ICS/University of Groningen

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks.

Original language	English
Pages (from-to)	7517-7520
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	61
DOIs	https://doi.org/10.1039/d1cc02290c
Publication status	Published - 7 Aug 2021

Access to Document

10.1039/d1cc02290c

Cite this

@article{4f9a4c48c72442c8987113fefb1d57b7,

title = "RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling",

abstract = "We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks.",

author = "Cyrille Jeancolas and Matsubara, \{Yoshiya J.\} and Mykhailo Vybornyi and Lambert, \{Camille N.\} and Alex Blokhuis and Thomas Alline and Griffiths, \{Andrew D.\} and Sandeep Ameta and Sandeep Krishna and Philippe Nghe",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = aug,

day = "7",

doi = "10.1039/d1cc02290c",

language = "English",

volume = "57",

pages = "7517--7520",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "61",

}

TY - JOUR

T1 - RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling

AU - Jeancolas, Cyrille

AU - Matsubara, Yoshiya J.

AU - Vybornyi, Mykhailo

AU - Lambert, Camille N.

AU - Blokhuis, Alex

AU - Alline, Thomas

AU - Griffiths, Andrew D.

AU - Ameta, Sandeep

AU - Krishna, Sandeep

AU - Nghe, Philippe

PY - 2021/8/7

Y1 - 2021/8/7

N2 - We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks.

AB - We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks.

UR - https://www.scopus.com/pages/publications/85111555159

U2 - 10.1039/d1cc02290c

DO - 10.1039/d1cc02290c

M3 - Article

C2 - 34235521

AN - SCOPUS:85111555159

SN - 1359-7345

VL - 57

SP - 7517

EP - 7520

JO - Chemical Communications

JF - Chemical Communications

IS - 61

ER -

RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling

Abstract

Access to Document

Other files and links

Fingerprint

Cite this