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Rod-derived cone viability factor promotes cone survival by stimulating aerobic glycolysis

  • Najate Aït-Ali
  • , Ram Fridlich
  • , Géraldine Millet-Puel
  • , Emmanuelle Clérin
  • , François Delalande
  • , Céline Jaillard
  • , Frédéric Blond
  • , Ludivine Perrocheau
  • , Sacha Reichman
  • , Leah C. Byrne
  • , Anne Olivier-Bandini
  • , Jacques Bellalou
  • , Emmanuel Moyse
  • , Frédéric Bouillaud
  • , Xavier Nicol
  • , Deniz Dalkara
  • , Alain Van Dorsselaer
  • , José Alain Sahel
  • , Thierry Léveillard

Research output: Contribution to journalArticlepeer-review

Abstract

Rod-derived cone viability factor (RdCVF) is an inactive thioredoxin secreted by rod photoreceptors that protects cones from degeneration. Because the secondary loss of cones in retinitis pigmentosa (RP) leads to blindness, the administration of RdCVF is a promising therapy for this untreatable neurodegenerative disease. Here, we investigated the mechanism underlying the protective role of RdCVF in RP. We show that RdCVF acts through binding to Basigin-1 (BSG1), a transmembrane protein expressed specifically by photoreceptors. BSG1 binds to the glucose transporter GLUT1, resulting in increased glucose entry into cones. Increased glucose promotes cone survival by stimulation of aerobic glycolysis. Moreover, a missense mutation of RdCVF results in its inability to bind to BSG1, stimulate glucose uptake, and prevent secondary cone death in a model of RP. Our data uncover an entirely novel mechanism of neuroprotection through the stimulation of glucose metabolism.

Original languageEnglish
Pages (from-to)817-832
Number of pages16
JournalCell
Volume161
Issue number4
DOIs
Publication statusPublished - 7 May 2015

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