Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G·C·G·C tetrad

Kah Wai Lim; Patrizia Alberti; Aurore Guédin; Laurent Lacroix; Jean François Riou; Nicola J. Royle; Jean Louis Mergny; Anh Tuân Phan

doi:10.1093/nar/gkp630

Sequence variant (CTAGGG)_n in the human telomere favors a G-quadruplex structure containing a G·C·G·C tetrad

Kah Wai Lim
, Patrizia Alberti
, Aurore Guédin
, Laurent Lacroix
, Jean François Riou
, Nicola J. Royle
, Jean Louis Mergny
, Anh Tuân Phan

Research output: Contribution to journal › Article › peer-review

Abstract

Short contiguous arrays of variant CTAGGG repeats in the human telomere are unstable in the male germline and somatic cells, suggesting formation of unusual structures by this repeat type. Here, we report on the structure of an intramolecular G-quadruplex formed by DNA sequences containing four human telomeric variant CTAGGG repeats in potassium solution. Our results reveal a new robust antiparallel G-quadruplex fold involving two G-tetrads sandwiched between a G·C base pair and a G·C·G·C tetrad, which could represent a new platform for drug design targeted to human telomeric DNA.

Original language	English
Pages (from-to)	6239-6248
Number of pages	10
Journal	Nucleic Acids Research
Volume	37
Issue number	18
DOIs	https://doi.org/10.1093/nar/gkp630
Publication status	Published - 9 Nov 2009
Externally published	Yes

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10.1093/nar/gkp630

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title = "Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G·C·G·C tetrad",

abstract = "Short contiguous arrays of variant CTAGGG repeats in the human telomere are unstable in the male germline and somatic cells, suggesting formation of unusual structures by this repeat type. Here, we report on the structure of an intramolecular G-quadruplex formed by DNA sequences containing four human telomeric variant CTAGGG repeats in potassium solution. Our results reveal a new robust antiparallel G-quadruplex fold involving two G-tetrads sandwiched between a G·C base pair and a G·C·G·C tetrad, which could represent a new platform for drug design targeted to human telomeric DNA.",

author = "Lim, \{Kah Wai\} and Patrizia Alberti and Aurore Gu{\'e}din and Laurent Lacroix and Riou, \{Jean Fran{\c c}ois\} and Royle, \{Nicola J.\} and Mergny, \{Jean Louis\} and Phan, \{Anh Tu{\^a}n\}",

note = "Funding Information: Singapore Biomedical Research Council (grant 07/1/22/ 19/542 to A.T.P.); Singapore Ministry of Education (ARC30/07 and RG62/07 to A.T.P.); Nanyang",

year = "2009",

month = nov,

day = "9",

doi = "10.1093/nar/gkp630",

language = "English",

volume = "37",

pages = "6239--6248",

journal = "Nucleic Acids Research",

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TY - JOUR

T1 - Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G·C·G·C tetrad

AU - Lim, Kah Wai

AU - Alberti, Patrizia

AU - Guédin, Aurore

AU - Lacroix, Laurent

AU - Riou, Jean François

AU - Royle, Nicola J.

AU - Mergny, Jean Louis

AU - Phan, Anh Tuân

N1 - Funding Information: Singapore Biomedical Research Council (grant 07/1/22/ 19/542 to A.T.P.); Singapore Ministry of Education (ARC30/07 and RG62/07 to A.T.P.); Nanyang

PY - 2009/11/9

Y1 - 2009/11/9

N2 - Short contiguous arrays of variant CTAGGG repeats in the human telomere are unstable in the male germline and somatic cells, suggesting formation of unusual structures by this repeat type. Here, we report on the structure of an intramolecular G-quadruplex formed by DNA sequences containing four human telomeric variant CTAGGG repeats in potassium solution. Our results reveal a new robust antiparallel G-quadruplex fold involving two G-tetrads sandwiched between a G·C base pair and a G·C·G·C tetrad, which could represent a new platform for drug design targeted to human telomeric DNA.

AB - Short contiguous arrays of variant CTAGGG repeats in the human telomere are unstable in the male germline and somatic cells, suggesting formation of unusual structures by this repeat type. Here, we report on the structure of an intramolecular G-quadruplex formed by DNA sequences containing four human telomeric variant CTAGGG repeats in potassium solution. Our results reveal a new robust antiparallel G-quadruplex fold involving two G-tetrads sandwiched between a G·C base pair and a G·C·G·C tetrad, which could represent a new platform for drug design targeted to human telomeric DNA.

U2 - 10.1093/nar/gkp630

DO - 10.1093/nar/gkp630

M3 - Article

C2 - 19692585

AN - SCOPUS:70350692142

SN - 0305-1048

VL - 37

SP - 6239

EP - 6248

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 18

ER -

Sequence variant (CTAGGG)n in the human telomere favors a G-quadruplex structure containing a G·C·G·C tetrad

Abstract

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Sequence variant (CTAGGG)_n in the human telomere favors a G-quadruplex structure containing a G·C·G·C tetrad