Single quantum dot imaging in live cells: Towards a cellular GPS

  • Sébastien Courty
  • , Marcel Zevenbergen
  • , Cédric Bouzigues
  • , Marie Virginie Ehrensperger
  • , Camilla Luccardini
  • , Assa Sittner
  • , Stéphane Bonneau
  • , Maxime Dahan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Colloidal semiconductor quantum dots (QDs) have become common fluorescent probes in biology. Their optical properties not only facilitate spectrally multiplexed detection but also enable single molecule measurements with high signal to noise ratio. This is of particular interest in cell biology since it allows individual QD-tagged biomolecules to be tracked with good spatial and temporal resolution over long durations. Recent measurements on membrane proteins have validated this approach and serve as a basis for more complex experiments in which the motion of different biomolecules, located in various cell compartments (membrane, cytosol, nucleus,...) and tagged with QDs having distinct emission colors, is recorded in real time and with a nanometer resolution. The development of these new imaging methods, equivalent to a molecular positioning system within a single cell, raises many challenges, coming from optics, physical and biological chemistry, as well as image processing.

Original languageEnglish
Title of host publicationColloidal Quantum Dots for Biomedical Applications
DOIs
Publication statusPublished - 26 Jun 2006
Externally publishedYes
EventColloidal Quantum Dots for Biomedical Applications - San Jose, CA, United States
Duration: 22 Jan 200624 Jan 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6096
ISSN (Print)1605-7422

Conference

ConferenceColloidal Quantum Dots for Biomedical Applications
Country/TerritoryUnited States
CitySan Jose, CA
Period22/01/0624/01/06

Keywords

  • Colloids
  • Quantum dots
  • Single molecule
  • Structured light illumination

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