SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation

Oriol Ros; Sarah Baudet; Yvrick Zagar; Karine Loulier; Fiona Roche; Sandrine Couvet; Alain Aghaie; Melody Atkins; Alice Louail; Christine Petit; Christine Metin; Yves Mechulam; Xavier Nicol

doi:10.1016/j.celrep.2020.107934

SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation

Oriol Ros
, Sarah Baudet
, Yvrick Zagar
, Karine Loulier
, Fiona Roche
, Sandrine Couvet
, Alain Aghaie
, Melody Atkins
, Alice Louail
, Christine Petit
, Christine Metin
, Yves Mechulam
, Xavier Nicol

Research output: Contribution to journal › Article › peer-review

Abstract

Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a versatile and genetically encoded chelator combining low- and high-affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.

Original language	English
Article number	107934
Journal	Cell Reports
Volume	32
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2020.107934
Publication status	Published - 21 Jul 2020

Keywords

EF hand
axon guidance
calcium
calcium buffer
calmodulin
neuronal migration
parvalbumin
single-cell pharmacology
subcellular calcium manipulation
subcellular compartment

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10.1016/j.celrep.2020.107934

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title = "SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation",

abstract = "Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a versatile and genetically encoded chelator combining low- and high-affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.",

keywords = "EF hand, axon guidance, calcium, calcium buffer, calmodulin, neuronal migration, parvalbumin, single-cell pharmacology, subcellular calcium manipulation, subcellular compartment",

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doi = "10.1016/j.celrep.2020.107934",

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AU - Ros, Oriol

AU - Baudet, Sarah

AU - Zagar, Yvrick

AU - Loulier, Karine

AU - Roche, Fiona

AU - Couvet, Sandrine

AU - Aghaie, Alain

AU - Atkins, Melody

AU - Louail, Alice

AU - Petit, Christine

AU - Metin, Christine

AU - Mechulam, Yves

AU - Nicol, Xavier

PY - 2020/7/21

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AB - Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a versatile and genetically encoded chelator combining low- and high-affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.

KW - EF hand

KW - axon guidance

KW - calcium

KW - calcium buffer

KW - calmodulin

KW - neuronal migration

KW - parvalbumin

KW - single-cell pharmacology

KW - subcellular calcium manipulation

KW - subcellular compartment

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DO - 10.1016/j.celrep.2020.107934

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JO - Cell Reports

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ER -

SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation

Abstract

Keywords

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