Stimulated emission depletion microscopy to study amyloid fibril formation

  • Pierre Mahou
  • , Nathan Curry
  • , Dorothea Pinotsi
  • , Gabriele Kaminski Schierle
  • , Clemens Kaminski

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Aggregation of misfolded proteins is a characteristic hallmark of many neurodegenerative disorders, such as Parkinson’s, Alzheimer’s and Huntington’s diseases. The ability to observe these aggregation processes and the corresponding structures formed in vitro or in situ is therefore a key requirement to understand the molecular mechanisms of these diseases. We report here on the implementation and application of Stimulated Emission Depletion (STED) microscopy to visualize the formation of amyloid fibrils in vitro.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging VIII
EditorsIngo Gregor, Zygmunt Karol Gryczynski, Felix Koberling, Jorg Enderlein, Rainer Erdmann
PublisherSPIE
ISBN (Electronic)9781628414219
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes
EventSingle Molecule Spectroscopy and Superresolution Imaging VIII - San Francisco, United States
Duration: 7 Feb 20158 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9331
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging VIII
Country/TerritoryUnited States
CitySan Francisco
Period7/02/158/02/15

Keywords

  • Stimulated Emission Depletion microscopy
  • Super resolution microscopy
  • amyloid fibrils

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