Study by ³¹P NMR spectroscopy of the triacylglycerol degradation processes in olive oil with different heat-transfer mechanisms

The thermal degradation of olive oil using conventional and microwave heating under the same experimental conditions were compared. A powerful identification and quantification technique based on ³¹P NMR has been developed to characterise the differences between the minor components including diacylglycerol and free fatty acids in the heated samples. The ³¹P NMR spectra of the degraded olive oils, which contain OH groups derivatised with a phosphorus reagent, showed that conventional heating is more detrimental to the oil than microwave technique. Conventional heating leads to a significant increase in the diacylglycerol and free fatty acid contents as well as in the number of degradation compounds, which damage the olive oil quality. However, the main process that takes place on using microwave heating is isomerisation between diacylglycerols, a change that could give a potential longer shelf life to the olive oil.