Tetramolecular quadruplex stability and assembly

Phong Lan Thao Tran, Anne De Cian, Julien Gros, Rui Moriyama, Jean Louis Mergny

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Guanine quadruplexes (G4) are unusual four-stranded nucleic acid structures formed by G-rich DNA/RNA. Beyond their likely biological relevance, the self-assembly, stability, and rigidity of these structures are also interesting for nanotechnology and biotechnology applications. Therefore, efforts are carried out to understand the rules that govern stability and folding of G-quadruplexes. We focus this chapter on tetramolecular conformations which are simple tractable models. We report here the experimental parameters, molecules, and modifications that affect thermal stability and/or association kinetics of these structures. Some chemical modifications which facilitate tetramolecular quadruplex formation and can be useful for nano- or biotechnology are also described.

Original languageEnglish
Title of host publicationQuadruplex Nucleic Acids
EditorsJonathan B. Chaires, David Graves
Pages243-274
Number of pages32
DOIs
Publication statusPublished - 11 Apr 2013
Externally publishedYes

Publication series

NameTopics in Current Chemistry
Volume330
ISSN (Print)0340-1022

Keywords

  • Association kinetics
  • Chemical modifications
  • Nanotechnology and biotechnology applications
  • Tetramolecular quadruplexes
  • Thermal stability

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