The membrane-extrinsic domain of cytochrome b558/566 from the Archaeon Sulfolobus acidocaldarius performs pivoting movements with respect to the membrane surface

Barbara Schoepp-Cothenet; Michael Schütz; Frauke Baymann; Myriam Brugna; Wolfgang Nitschke; Hannu Myllykallio; Christian Schmidt

doi:10.1016/S0014-5793(00)02357-7

The membrane-extrinsic domain of cytochrome b_558/566 from the Archaeon Sulfolobus acidocaldarius performs pivoting movements with respect to the membrane surface

Barbara Schoepp-Cothenet
, Michael Schütz
, Frauke Baymann
, Myriam Brugna
, Wolfgang Nitschke
, Hannu Myllykallio
, Christian Schmidt

Research output: Contribution to journal › Article › peer-review

Abstract

The orientation of the membrane-attached cytochrome b_558/566-haem with respect to the membrane was determined by electron paramagnetic resonance spectroscopy on two-dimensionally ordered oxidised membrane fragments from Sulfolobus acidocaldarius. Unlike the other redox centres in the membrane, the cytochrome b_558/566-haem was found to cover a range of orientations between 25° and 90°. The described results are reminiscent of those obtained on the Rieske cluster of bc complexes and indicate that the membrane-extrinsic domain of cytochrome b_558/566 can perform pivoting motion between two extreme positions. Such a conformational flexibility is likely to play a role in electron transfer with its redox partners.

Original language	English
Pages (from-to)	372-376
Number of pages	5
Journal	FEBS Letters
Volume	487
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(00)02357-7
Publication status	Published - 5 Jan 2001

Keywords

Archaeon
Cytochrome b
Cytochrome c
Electron paramagnetic resonance
Sulfolobus

Access to Document

10.1016/S0014-5793(00)02357-7

Cite this

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title = "The membrane-extrinsic domain of cytochrome b558/566 from the Archaeon Sulfolobus acidocaldarius performs pivoting movements with respect to the membrane surface",

abstract = "The orientation of the membrane-attached cytochrome b558/566-haem with respect to the membrane was determined by electron paramagnetic resonance spectroscopy on two-dimensionally ordered oxidised membrane fragments from Sulfolobus acidocaldarius. Unlike the other redox centres in the membrane, the cytochrome b558/566-haem was found to cover a range of orientations between 25° and 90°. The described results are reminiscent of those obtained on the Rieske cluster of bc complexes and indicate that the membrane-extrinsic domain of cytochrome b558/566 can perform pivoting motion between two extreme positions. Such a conformational flexibility is likely to play a role in electron transfer with its redox partners.",

keywords = "Archaeon, Cytochrome b, Cytochrome c, Electron paramagnetic resonance, Sulfolobus",

author = "Barbara Schoepp-Cothenet and Michael Sch{\"u}tz and Frauke Baymann and Myriam Brugna and Wolfgang Nitschke and Hannu Myllykallio and Christian Schmidt",

year = "2001",

month = jan,

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doi = "10.1016/S0014-5793(00)02357-7",

language = "English",

volume = "487",

pages = "372--376",

journal = "FEBS Letters",

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TY - JOUR

T1 - The membrane-extrinsic domain of cytochrome b558/566 from the Archaeon Sulfolobus acidocaldarius performs pivoting movements with respect to the membrane surface

AU - Schoepp-Cothenet, Barbara

AU - Schütz, Michael

AU - Baymann, Frauke

AU - Brugna, Myriam

AU - Nitschke, Wolfgang

AU - Myllykallio, Hannu

AU - Schmidt, Christian

PY - 2001/1/5

Y1 - 2001/1/5

N2 - The orientation of the membrane-attached cytochrome b558/566-haem with respect to the membrane was determined by electron paramagnetic resonance spectroscopy on two-dimensionally ordered oxidised membrane fragments from Sulfolobus acidocaldarius. Unlike the other redox centres in the membrane, the cytochrome b558/566-haem was found to cover a range of orientations between 25° and 90°. The described results are reminiscent of those obtained on the Rieske cluster of bc complexes and indicate that the membrane-extrinsic domain of cytochrome b558/566 can perform pivoting motion between two extreme positions. Such a conformational flexibility is likely to play a role in electron transfer with its redox partners.

AB - The orientation of the membrane-attached cytochrome b558/566-haem with respect to the membrane was determined by electron paramagnetic resonance spectroscopy on two-dimensionally ordered oxidised membrane fragments from Sulfolobus acidocaldarius. Unlike the other redox centres in the membrane, the cytochrome b558/566-haem was found to cover a range of orientations between 25° and 90°. The described results are reminiscent of those obtained on the Rieske cluster of bc complexes and indicate that the membrane-extrinsic domain of cytochrome b558/566 can perform pivoting motion between two extreme positions. Such a conformational flexibility is likely to play a role in electron transfer with its redox partners.

KW - Archaeon

KW - Cytochrome b

KW - Cytochrome c

KW - Electron paramagnetic resonance

KW - Sulfolobus

U2 - 10.1016/S0014-5793(00)02357-7

DO - 10.1016/S0014-5793(00)02357-7

M3 - Article

C2 - 11163361

AN - SCOPUS:0035808256

SN - 0014-5793

VL - 487

SP - 372

EP - 376

JO - FEBS Letters

JF - FEBS Letters

IS - 3