The Paris-Sud yeast structural genomics pilot-project: From structure to function

Sophie Quevillon-Cheruel; Dominique Liger; Nicolas Leulliot; Marc Graille; Anne Poupon; Inès Li De La Sierra-Gallay; Cong Zhao Zhou; Bruno Collinet; Joël Janin; Herman Van Tilbeurgh

doi:10.1016/j.biochi.2004.09.013

The Paris-Sud yeast structural genomics pilot-project: From structure to function

Sophie Quevillon-Cheruel
, Dominique Liger
, Nicolas Leulliot
, Marc Graille
, Anne Poupon
, Inès Li De La Sierra-Gallay
, Cong Zhao Zhou
, Bruno Collinet
, Joël Janin
, Herman Van Tilbeurgh

Inst. Biochim. Biophysique Molec. C.
Université Paris-Saclay
Laboratoi Re d'Enzymol. Biochim. S.
Bâtiment 34
1, Ave. Terrasse, 91198 Gif Sur Y.

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We present here the outlines and results from our yeast structural genomics (YSG) pilot-project. A lab-scale platform for the systematic production and structure determination is presented. In order to validate this approach, 250 non-membrane proteins of unknown structure were targeted. Strategies and final statistics are evaluated. We finally discuss the opportunity of structural genomics programs to contribute to functional biochemical annotation.

Original language	English
Pages (from-to)	617-623
Number of pages	7
Journal	Biochimie
Volume	86
Issue number	9-10
DOIs	https://doi.org/10.1016/j.biochi.2004.09.013
Publication status	Published - 1 Sept 2004
Externally published	Yes

Keywords

Crystallization robotics
Crystallography
Expression
Platform

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10.1016/j.biochi.2004.09.013

Cite this

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title = "The Paris-Sud yeast structural genomics pilot-project: From structure to function",

abstract = "We present here the outlines and results from our yeast structural genomics (YSG) pilot-project. A lab-scale platform for the systematic production and structure determination is presented. In order to validate this approach, 250 non-membrane proteins of unknown structure were targeted. Strategies and final statistics are evaluated. We finally discuss the opportunity of structural genomics programs to contribute to functional biochemical annotation.",

keywords = "Crystallization robotics, Crystallography, Expression, Platform",

author = "Sophie Quevillon-Cheruel and Dominique Liger and Nicolas Leulliot and Marc Graille and Anne Poupon and \{De La Sierra-Gallay\}, \{In{\`e}s Li\} and Zhou, \{Cong Zhao\} and Bruno Collinet and Jo{\"e}l Janin and \{Van Tilbeurgh\}, Herman",

year = "2004",

month = sep,

day = "1",

doi = "10.1016/j.biochi.2004.09.013",

language = "English",

volume = "86",

pages = "617--623",

journal = "Biochimie",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - The Paris-Sud yeast structural genomics pilot-project

T2 - From structure to function

AU - Quevillon-Cheruel, Sophie

AU - Liger, Dominique

AU - Leulliot, Nicolas

AU - Graille, Marc

AU - Poupon, Anne

AU - De La Sierra-Gallay, Inès Li

AU - Zhou, Cong Zhao

AU - Collinet, Bruno

AU - Janin, Joël

AU - Van Tilbeurgh, Herman

PY - 2004/9/1

Y1 - 2004/9/1

N2 - We present here the outlines and results from our yeast structural genomics (YSG) pilot-project. A lab-scale platform for the systematic production and structure determination is presented. In order to validate this approach, 250 non-membrane proteins of unknown structure were targeted. Strategies and final statistics are evaluated. We finally discuss the opportunity of structural genomics programs to contribute to functional biochemical annotation.

AB - We present here the outlines and results from our yeast structural genomics (YSG) pilot-project. A lab-scale platform for the systematic production and structure determination is presented. In order to validate this approach, 250 non-membrane proteins of unknown structure were targeted. Strategies and final statistics are evaluated. We finally discuss the opportunity of structural genomics programs to contribute to functional biochemical annotation.

KW - Crystallization robotics

KW - Crystallography

KW - Expression

KW - Platform

U2 - 10.1016/j.biochi.2004.09.013

DO - 10.1016/j.biochi.2004.09.013

M3 - Article

C2 - 15556271

AN - SCOPUS:8844246397

SN - 0300-9084

VL - 86

SP - 617

EP - 623

JO - Biochimie

JF - Biochimie

IS - 9-10

ER -

The Paris-Sud yeast structural genomics pilot-project: From structure to function

Abstract

Keywords

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