The Paris-Sud yeast structural genomics pilot-project: From structure to function

  • Sophie Quevillon-Cheruel
  • , Dominique Liger
  • , Nicolas Leulliot
  • , Marc Graille
  • , Anne Poupon
  • , Inès Li De La Sierra-Gallay
  • , Cong Zhao Zhou
  • , Bruno Collinet
  • , Joël Janin
  • , Herman Van Tilbeurgh

Research output: Contribution to journalArticlepeer-review

Abstract

We present here the outlines and results from our yeast structural genomics (YSG) pilot-project. A lab-scale platform for the systematic production and structure determination is presented. In order to validate this approach, 250 non-membrane proteins of unknown structure were targeted. Strategies and final statistics are evaluated. We finally discuss the opportunity of structural genomics programs to contribute to functional biochemical annotation.

Original languageEnglish
Pages (from-to)617-623
Number of pages7
JournalBiochimie
Volume86
Issue number9-10
DOIs
Publication statusPublished - 1 Sept 2004
Externally publishedYes

Keywords

  • Crystallization robotics
  • Crystallography
  • Expression
  • Platform

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