Thermodynamical and structural information on photosynthetic systems obtained from electroluminescence kinetics

Marten H. Vos; Hans J. van Gorkom

doi:10.1016/S0006-3495(90)82499-1

Thermodynamical and structural information on photosynthetic systems obtained from electroluminescence kinetics

Marten H. Vos
, Hans J. van Gorkom

University of Leiden

Research output: Contribution to journal › Article › peer-review

Abstract

The relationship between the thermodynamical and structural properties of photosynthetic reaction centers and kinetics and polarization of electric field-induced luminescence was studied. A general model is presented to describe the influence of an electric field on the individual electron transfer rate constants. Comparison of simulations with this model and experimental curves of Photosystem I electroluminescence showed that (a) at least three electrogenic electron transfer steps occur: P-700 to A₀(∼30%), A₀ to A₁ (∼50%), and A₁ to F_A(∼20%), (b) the midpoint potential of A₁/A-₁ is ∼ - 0.81 V, and (c) the emission moments of the pigments make on average an angle of 67° with the membrane normal. It is concluded that the analysis of electro-luminescence kinetics may be a powerful technique to obtain information on primary processes using relatively intact systems.

Original language	English
Pages (from-to)	1547-1555
Number of pages	9
Journal	Biophysical Journal
Volume	58
Issue number	6
DOIs	https://doi.org/10.1016/S0006-3495(90)82499-1
Publication status	Published - 1 Jan 1990
Externally published	Yes

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10.1016/S0006-3495(90)82499-1

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title = "Thermodynamical and structural information on photosynthetic systems obtained from electroluminescence kinetics",

abstract = "The relationship between the thermodynamical and structural properties of photosynthetic reaction centers and kinetics and polarization of electric field-induced luminescence was studied. A general model is presented to describe the influence of an electric field on the individual electron transfer rate constants. Comparison of simulations with this model and experimental curves of Photosystem I electroluminescence showed that (a) at least three electrogenic electron transfer steps occur: P-700 to A0(∼30\%), A0 to A1 (∼50\%), and A1 to FA(∼20\%), (b) the midpoint potential of A1/A-1 is ∼ - 0.81 V, and (c) the emission moments of the pigments make on average an angle of 67° with the membrane normal. It is concluded that the analysis of electro-luminescence kinetics may be a powerful technique to obtain information on primary processes using relatively intact systems.",

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year = "1990",

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T1 - Thermodynamical and structural information on photosynthetic systems obtained from electroluminescence kinetics

AU - Vos, Marten H.

AU - van Gorkom, Hans J.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The relationship between the thermodynamical and structural properties of photosynthetic reaction centers and kinetics and polarization of electric field-induced luminescence was studied. A general model is presented to describe the influence of an electric field on the individual electron transfer rate constants. Comparison of simulations with this model and experimental curves of Photosystem I electroluminescence showed that (a) at least three electrogenic electron transfer steps occur: P-700 to A0(∼30%), A0 to A1 (∼50%), and A1 to FA(∼20%), (b) the midpoint potential of A1/A-1 is ∼ - 0.81 V, and (c) the emission moments of the pigments make on average an angle of 67° with the membrane normal. It is concluded that the analysis of electro-luminescence kinetics may be a powerful technique to obtain information on primary processes using relatively intact systems.

AB - The relationship between the thermodynamical and structural properties of photosynthetic reaction centers and kinetics and polarization of electric field-induced luminescence was studied. A general model is presented to describe the influence of an electric field on the individual electron transfer rate constants. Comparison of simulations with this model and experimental curves of Photosystem I electroluminescence showed that (a) at least three electrogenic electron transfer steps occur: P-700 to A0(∼30%), A0 to A1 (∼50%), and A1 to FA(∼20%), (b) the midpoint potential of A1/A-1 is ∼ - 0.81 V, and (c) the emission moments of the pigments make on average an angle of 67° with the membrane normal. It is concluded that the analysis of electro-luminescence kinetics may be a powerful technique to obtain information on primary processes using relatively intact systems.

U2 - 10.1016/S0006-3495(90)82499-1

DO - 10.1016/S0006-3495(90)82499-1

M3 - Article

AN - SCOPUS:0025666994

SN - 0006-3495

VL - 58

SP - 1547

EP - 1555

JO - Biophysical Journal

JF - Biophysical Journal

IS - 6

ER -

Thermodynamical and structural information on photosynthetic systems obtained from electroluminescence kinetics

Abstract

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