Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

Irene Costantini; Giacomo Mazzamuto; Annunziatina Laurino; Erica Lazzeri; Leonardo Sacconi; Mattia Neri; Andrea Simonetto; Matteo Roffilli; Ludovico Silvestri; Francesco S. Pavone

doi:10.1117/12.2527096

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

Irene Costantini
, Giacomo Mazzamuto
, Annunziatina Laurino
, Erica Lazzeri
, Leonardo Sacconi
, Mattia Neri
, Andrea Simonetto
, Matteo Roffilli
, Ludovico Silvestri
, Francesco S. Pavone

LENS, University of Florence
LENS
University of Florence
Bioretics Srl

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.

Original language	English
Title of host publication	Advances in Microscopic Imaging II
Editors	Emmanuel Beaurepaire, Francesco Saverio Pavone
Publisher	SPIE
ISBN (Electronic)	9781510628458
DOIs	https://doi.org/10.1117/12.2527096
Publication status	Published - 1 Jan 2019
Externally published	Yes
Event	Advances in Microscopic Imaging II 2019 - Munich, Germany Duration: 26 Jun 2019 → 27 Jun 2019

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11076
ISSN (Print)	1605-7422

Conference

Conference	Advances in Microscopic Imaging II 2019
Country/Territory	Germany
City	Munich
Period	26/06/19 → 27/06/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

3D imaging
Brain
Clearing
Human
SWITCH
TDE

Access to Document

10.1117/12.2527096

Cite this

Costantini, I., Mazzamuto, G., Laurino, A., Lazzeri, E., Sacconi, L., Neri, M., Simonetto, A., Roffilli, M., Silvestri, L., & Pavone, F. S. (2019). Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. In E. Beaurepaire, & F. S. Pavone (Eds.), Advances in Microscopic Imaging II Article 110760B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11076). SPIE. https://doi.org/10.1117/12.2527096

@inproceedings{f2dfa43a1654470c866f3da967f55870,

title = "Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method",

abstract = "The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.",

keywords = "3D imaging, Brain, Clearing, Human, SWITCH, TDE",

author = "Irene Costantini and Giacomo Mazzamuto and Annunziatina Laurino and Erica Lazzeri and Leonardo Sacconi and Mattia Neri and Andrea Simonetto and Matteo Roffilli and Ludovico Silvestri and Pavone, \{Francesco S.\}",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Advances in Microscopic Imaging II 2019 ; Conference date: 26-06-2019 Through 27-06-2019",

year = "2019",

month = jan,

day = "1",

doi = "10.1117/12.2527096",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

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Costantini, I, Mazzamuto, G, Laurino, A, Lazzeri, E, Sacconi, L, Neri, M, Simonetto, A, Roffilli, M, Silvestri, L & Pavone, FS 2019, Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. in E Beaurepaire & FS Pavone (eds), Advances in Microscopic Imaging II., 110760B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11076, SPIE, Advances in Microscopic Imaging II 2019, Munich, Germany, 26/06/19. https://doi.org/10.1117/12.2527096

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. / Costantini, Irene; Mazzamuto, Giacomo; Laurino, Annunziatina et al.
Advances in Microscopic Imaging II. ed. / Emmanuel Beaurepaire; Francesco Saverio Pavone. SPIE, 2019. 110760B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11076).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

AU - Costantini, Irene

AU - Mazzamuto, Giacomo

AU - Laurino, Annunziatina

AU - Lazzeri, Erica

AU - Sacconi, Leonardo

AU - Neri, Mattia

AU - Simonetto, Andrea

AU - Roffilli, Matteo

AU - Silvestri, Ludovico

AU - Pavone, Francesco S.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.

AB - The three-dimensional reconstruction of large volumes of the human neural networks at cellular resolution is one of the biggest challenges of our days. Commonly, fine slices of samples marked with colorimetric techniques are individually imaged. This approach in addition to being time-consuming does not consider space cell organization, leading to loss of information. The aim of this work was to develop a methodology that allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. In particular, we exploit the possibility of combining high-resolution 3D imaging techniques with clearing methodologies. We successfully integrate the SWITCH immunohistochemistry technique with the TDE clearing method to image a large volume of human brain tissue with two-photon fluorescence microscopy. In conclusion, this new approach enables to characterize large human brain specimens with high-resolution optical techniques, giving the possibility to expand the histological studies to the third dimension.

KW - 3D imaging

KW - Brain

KW - Clearing

KW - Human

KW - SWITCH

KW - TDE

UR - https://www.scopus.com/pages/publications/85074301285

U2 - 10.1117/12.2527096

DO - 10.1117/12.2527096

M3 - Conference contribution

AN - SCOPUS:85074301285

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Advances in Microscopic Imaging II

A2 - Beaurepaire, Emmanuel

A2 - Pavone, Francesco Saverio

PB - SPIE

T2 - Advances in Microscopic Imaging II 2019

Y2 - 26 June 2019 through 27 June 2019

ER -

Costantini I, Mazzamuto G, Laurino A, Lazzeri E, Sacconi L, Neri M et al. Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method. In Beaurepaire E, Pavone FS, editors, Advances in Microscopic Imaging II. SPIE. 2019. 110760B. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2527096

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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