TY - GEN
T1 - Three-dimensional cell culture in droplets
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
AU - Sart, Sebastien
AU - Tomasi, Raphael F.X.
AU - Amselem, Gabriel
AU - Baroud, Charles N.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Three-dimensional (3D) cell cultures are rapidly emerging as a promising format to explore biological functions, for a wide range of applications such as drug screening, personalized cancer diagnostics, or tissue engineering for cellular therapy. 3D spheroids offer an attractive format in which the cells adhere together through the extracellular matrix that they secrete. However it is still impossible to acquire large sets of detailed measurements on the 3D cultures, such as those afforded by flow cytometry for individual cells, without breaking the spheroids. Here we present a microfluidic approach to form the spheroids and culture them on a single chip, by leveraging droplet microfluidic methods. The device allows the formation, culture, stimulation, and observation of several hundred spheroids on a single device, in addition to the retrieval of any of them for further off-chip processing. We show how to obtain population, spheroid, and cell-level measurements, as well as applications for drug screening and tissue engineering.
AB - Three-dimensional (3D) cell cultures are rapidly emerging as a promising format to explore biological functions, for a wide range of applications such as drug screening, personalized cancer diagnostics, or tissue engineering for cellular therapy. 3D spheroids offer an attractive format in which the cells adhere together through the extracellular matrix that they secrete. However it is still impossible to acquire large sets of detailed measurements on the 3D cultures, such as those afforded by flow cytometry for individual cells, without breaking the spheroids. Here we present a microfluidic approach to form the spheroids and culture them on a single chip, by leveraging droplet microfluidic methods. The device allows the formation, culture, stimulation, and observation of several hundred spheroids on a single device, in addition to the retrieval of any of them for further off-chip processing. We show how to obtain population, spheroid, and cell-level measurements, as well as applications for drug screening and tissue engineering.
KW - 3D cell culture
KW - Droplets
KW - Drug screening
KW - Hydrogels
M3 - Conference contribution
AN - SCOPUS:85014157450
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 53
EP - 54
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
Y2 - 9 October 2016 through 13 October 2016
ER -
