Tissue Molecular Ion Imaging by Gold Cluster Ion Bombardment

David Touboul; Fréderic Halgand; Alain Brunelle; Reinhard Kersting; Elke Tallarek; Birgit Hagenhoff; Olivier Laprévote

doi:10.1021/ac035243z

Tissue Molecular Ion Imaging by Gold Cluster Ion Bombardment

David Touboul
, Fréderic Halgand
, Alain Brunelle
, Reinhard Kersting
, Elke Tallarek
, Birgit Hagenhoff
, Olivier Laprévote

Centre national de la recherche scientifique
Tascon GmbH

Research output: Contribution to journal › Article › peer-review

Abstract

The use of gold cluster focused ion beams produced by a liquid metal ion gun in a TOF-SIMS mass spectrometer is shown to dramatically enhance secondary ion emission of phospholipids and peptides. The method has been successfully tested with cells grown onto plastic slips and with mouse brain slices, without any treatment of the samples. Very reliable time-of-flight mass spectra are acquired with a low primary ion dose of a few 10⁷ ions, and high lateral resolution molecular ion images are obtained for heavy ions of great biological interest. This approach offers new opportunities in pharmacological and biological research fields by localizing compounds of interest such as drugs or metabolites in tissues.

Original language	English
Pages (from-to)	1550-1559
Number of pages	10
Journal	Analytical Chemistry
Volume	76
Issue number	6
DOIs	https://doi.org/10.1021/ac035243z
Publication status	Published - 15 Mar 2004

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10.1021/ac035243z

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abstract = "The use of gold cluster focused ion beams produced by a liquid metal ion gun in a TOF-SIMS mass spectrometer is shown to dramatically enhance secondary ion emission of phospholipids and peptides. The method has been successfully tested with cells grown onto plastic slips and with mouse brain slices, without any treatment of the samples. Very reliable time-of-flight mass spectra are acquired with a low primary ion dose of a few 107 ions, and high lateral resolution molecular ion images are obtained for heavy ions of great biological interest. This approach offers new opportunities in pharmacological and biological research fields by localizing compounds of interest such as drugs or metabolites in tissues.",

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AU - Touboul, David

AU - Halgand, Fréderic

AU - Brunelle, Alain

AU - Kersting, Reinhard

AU - Tallarek, Elke

AU - Hagenhoff, Birgit

AU - Laprévote, Olivier

PY - 2004/3/15

Y1 - 2004/3/15

N2 - The use of gold cluster focused ion beams produced by a liquid metal ion gun in a TOF-SIMS mass spectrometer is shown to dramatically enhance secondary ion emission of phospholipids and peptides. The method has been successfully tested with cells grown onto plastic slips and with mouse brain slices, without any treatment of the samples. Very reliable time-of-flight mass spectra are acquired with a low primary ion dose of a few 107 ions, and high lateral resolution molecular ion images are obtained for heavy ions of great biological interest. This approach offers new opportunities in pharmacological and biological research fields by localizing compounds of interest such as drugs or metabolites in tissues.

AB - The use of gold cluster focused ion beams produced by a liquid metal ion gun in a TOF-SIMS mass spectrometer is shown to dramatically enhance secondary ion emission of phospholipids and peptides. The method has been successfully tested with cells grown onto plastic slips and with mouse brain slices, without any treatment of the samples. Very reliable time-of-flight mass spectra are acquired with a low primary ion dose of a few 107 ions, and high lateral resolution molecular ion images are obtained for heavy ions of great biological interest. This approach offers new opportunities in pharmacological and biological research fields by localizing compounds of interest such as drugs or metabolites in tissues.

U2 - 10.1021/ac035243z

DO - 10.1021/ac035243z

M3 - Article

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VL - 76

SP - 1550

EP - 1559

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 6

ER -

Tissue Molecular Ion Imaging by Gold Cluster Ion Bombardment

Abstract

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