TOF-SIMS imaging of lipids on rat brain sections

David Touboul; Alain Brunelle

doi:10.1007/978-1-4939-1357-2_3

TOF-SIMS imaging of lipids on rat brain sections

David Touboul
, Alain Brunelle

Centre national de la recherche scientifique

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Since several decades, secondary ion mass spectrometry (SIMS) coupled to time of flight (TOF) is used for atomic or small inorganic/organic fragments imaging on different materials. With the advent of polyatomic ion sources leading to a significant increase of sensitivity in combination with a reasonable spatial resolution (1-10 μm), TOF-SIMS is becoming a more and more popular analytical platform for MS imaging. Even if this technique is limited to small molecules (typically below 1,000 Da), it offers enough sensitivity to detect and locate various classes of lipids directly on the surface of tissue sections. This chapter is thus dedicated to the TOF-SIMS analysis of lipids in positive and negative ion modes on rat brain tissue sections using a bismuth cluster ion source.

Original language	English
Title of host publication	Mass Spectrometry Imaging of Small Molecules
Publisher	Springer Fachmedien
Pages	21-27
Number of pages	7
ISBN (Electronic)	9781493913572
ISBN (Print)	9781493913565
DOIs	https://doi.org/10.1007/978-1-4939-1357-2_3
Publication status	Published - 31 Oct 2014

Keywords

Lipid
Mass spectrometry imaging
Rat brain
Secondary ion mass spectrometry
Time-of-fight

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10.1007/978-1-4939-1357-2_3

Cite this

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title = "TOF-SIMS imaging of lipids on rat brain sections",

abstract = "Since several decades, secondary ion mass spectrometry (SIMS) coupled to time of flight (TOF) is used for atomic or small inorganic/organic fragments imaging on different materials. With the advent of polyatomic ion sources leading to a significant increase of sensitivity in combination with a reasonable spatial resolution (1-10 μm), TOF-SIMS is becoming a more and more popular analytical platform for MS imaging. Even if this technique is limited to small molecules (typically below 1,000 Da), it offers enough sensitivity to detect and locate various classes of lipids directly on the surface of tissue sections. This chapter is thus dedicated to the TOF-SIMS analysis of lipids in positive and negative ion modes on rat brain tissue sections using a bismuth cluster ion source.",

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T1 - TOF-SIMS imaging of lipids on rat brain sections

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AU - Brunelle, Alain

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

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Y1 - 2014/10/31

N2 - Since several decades, secondary ion mass spectrometry (SIMS) coupled to time of flight (TOF) is used for atomic or small inorganic/organic fragments imaging on different materials. With the advent of polyatomic ion sources leading to a significant increase of sensitivity in combination with a reasonable spatial resolution (1-10 μm), TOF-SIMS is becoming a more and more popular analytical platform for MS imaging. Even if this technique is limited to small molecules (typically below 1,000 Da), it offers enough sensitivity to detect and locate various classes of lipids directly on the surface of tissue sections. This chapter is thus dedicated to the TOF-SIMS analysis of lipids in positive and negative ion modes on rat brain tissue sections using a bismuth cluster ion source.

AB - Since several decades, secondary ion mass spectrometry (SIMS) coupled to time of flight (TOF) is used for atomic or small inorganic/organic fragments imaging on different materials. With the advent of polyatomic ion sources leading to a significant increase of sensitivity in combination with a reasonable spatial resolution (1-10 μm), TOF-SIMS is becoming a more and more popular analytical platform for MS imaging. Even if this technique is limited to small molecules (typically below 1,000 Da), it offers enough sensitivity to detect and locate various classes of lipids directly on the surface of tissue sections. This chapter is thus dedicated to the TOF-SIMS analysis of lipids in positive and negative ion modes on rat brain tissue sections using a bismuth cluster ion source.

KW - Lipid

KW - Mass spectrometry imaging

KW - Rat brain

KW - Secondary ion mass spectrometry

KW - Time-of-fight

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EP - 27

BT - Mass Spectrometry Imaging of Small Molecules

PB - Springer Fachmedien

ER -

TOF-SIMS imaging of lipids on rat brain sections

Abstract

Keywords

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