TY - JOUR
T1 - Transport of long neutral polymers in the semidilute regime through a protein nanopore
AU - Oukhaled, Abdel Ghani
AU - Biance, Anne Laure
AU - Pelta, Juan
AU - Auvray, Loic
AU - Bacri, Laurent
PY - 2012/2/22
Y1 - 2012/2/22
N2 - We investigate the entrance of single poly(ethylene glycol) chains into an I-hemolysin channel. We detect the frequency and duration of the current blockades induced by large neutral polymers, where chain radius is larger than pore diameter. In the semidilute regime, these chains pass only if the monomer concentration is larger than a well-defined threshold. Experiments are performed in a very large domain of concentration and molecular mass, up to 35% and 200kDa, respectively, which was previously unexplored. The variation of the dwell time as a function of molecular mass shows that the chains are extracted from the semidilute solution in contact with the pore by a reptation mechanism.
AB - We investigate the entrance of single poly(ethylene glycol) chains into an I-hemolysin channel. We detect the frequency and duration of the current blockades induced by large neutral polymers, where chain radius is larger than pore diameter. In the semidilute regime, these chains pass only if the monomer concentration is larger than a well-defined threshold. Experiments are performed in a very large domain of concentration and molecular mass, up to 35% and 200kDa, respectively, which was previously unexplored. The variation of the dwell time as a function of molecular mass shows that the chains are extracted from the semidilute solution in contact with the pore by a reptation mechanism.
U2 - 10.1103/PhysRevLett.108.088104
DO - 10.1103/PhysRevLett.108.088104
M3 - Article
AN - SCOPUS:84857620658
SN - 0031-9007
VL - 108
JO - Physical Review Letters
JF - Physical Review Letters
IS - 8
M1 - 088104
ER -