Triple helix structures: Sequence dependence, flexibility and mismatch effects

Jian Sheng Sun; Jean Louis Mergny; Thérèse Montenay Garestier; Richard Lavery; Claude Hélène

doi:10.1080/07391102.1991.10507925

Triple helix structures: Sequence dependence, flexibility and mismatch effects

Jian Sheng Sun
, Jean Louis Mergny
, Thérèse Montenay Garestier
, Richard Lavery
, Claude Hélène

CNRS/Museum National d'Histoire Naturelle/IRD/UPMC
Centre national de la recherche scientifique

Research output: Contribution to journal › Article › peer-review

Abstract

By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine, homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen- bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption.

Original language	English
Pages (from-to)	411-424
Number of pages	14
Journal	Journal of Biomolecular Structure and Dynamics
Volume	9
Issue number	3
DOIs	https://doi.org/10.1080/07391102.1991.10507925
Publication status	Published - 1 Jan 1991
Externally published	Yes

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10.1080/07391102.1991.10507925

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title = "Triple helix structures: Sequence dependence, flexibility and mismatch effects",

abstract = "By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine, homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen- bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption.",

author = "Sun, \{Jian Sheng\} and Mergny, \{Jean Louis\} and Garestier, \{Th{\'e}r{\`e}se Montenay\} and Richard Lavery and Claude H{\'e}l{\`e}ne",

note = "Funding Information: The work of J.L.M. was supported by a financial grant of the Institut de Formation Superieure Biomedicale and Rhone-Poulenc.",

year = "1991",

month = jan,

day = "1",

doi = "10.1080/07391102.1991.10507925",

language = "English",

volume = "9",

pages = "411--424",

journal = "Journal of Biomolecular Structure and Dynamics",

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TY - JOUR

T1 - Triple helix structures

T2 - Sequence dependence, flexibility and mismatch effects

AU - Sun, Jian Sheng

AU - Mergny, Jean Louis

AU - Garestier, Thérèse Montenay

AU - Lavery, Richard

AU - Hélène, Claude

N1 - Funding Information: The work of J.L.M. was supported by a financial grant of the Institut de Formation Superieure Biomedicale and Rhone-Poulenc.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine, homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen- bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption.

AB - By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine, homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen- bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption.

U2 - 10.1080/07391102.1991.10507925

DO - 10.1080/07391102.1991.10507925

M3 - Article

C2 - 1815635

AN - SCOPUS:0026317524

SN - 0739-1102

VL - 9

SP - 411

EP - 424

JO - Journal of Biomolecular Structure and Dynamics

JF - Journal of Biomolecular Structure and Dynamics

IS - 3

ER -

Triple helix structures: Sequence dependence, flexibility and mismatch effects

Abstract

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