UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: A simple, rapid and sensitive quantification method

David Touboul; Joanna Wdzieczak-Bakala; Olivier Laprévote; Maria Concepcion Garcia-Alvarez

doi:10.1039/c0ay00576b

UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: A simple, rapid and sensitive quantification method

David Touboul, Joanna Wdzieczak-Bakala, Olivier Laprévote, Maria Concepcion Garcia-Alvarez

Research output: Contribution to journal › Article › peer-review

Abstract

Fast and reproducible quantification of thymosins β4 and β10 in different cell cultures was achieved by ultra high performance liquid chromatography coupled to mass spectrometry. We demonstrated that cancer cell lines all exhibit a higher amount of Tβ10 compared to control cells, whereas the level of Tβ4 is drastically depending on cell lines.

Original language	English
Pages (from-to)	678-682
Number of pages	5
Journal	Analytical Methods
Volume	3
Issue number	3
DOIs	https://doi.org/10.1039/c0ay00576b
Publication status	Published - 1 Mar 2011

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title = "UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: A simple, rapid and sensitive quantification method",

abstract = "Fast and reproducible quantification of thymosins β4 and β10 in different cell cultures was achieved by ultra high performance liquid chromatography coupled to mass spectrometry. We demonstrated that cancer cell lines all exhibit a higher amount of Tβ10 compared to control cells, whereas the level of Tβ4 is drastically depending on cell lines.",

author = "David Touboul and Joanna Wdzieczak-Bakala and Olivier Lapr{\'e}vote and Garcia-Alvarez, \{Maria Concepcion\}",

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AU - Touboul, David

AU - Wdzieczak-Bakala, Joanna

AU - Laprévote, Olivier

AU - Garcia-Alvarez, Maria Concepcion

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N2 - Fast and reproducible quantification of thymosins β4 and β10 in different cell cultures was achieved by ultra high performance liquid chromatography coupled to mass spectrometry. We demonstrated that cancer cell lines all exhibit a higher amount of Tβ10 compared to control cells, whereas the level of Tβ4 is drastically depending on cell lines.

AB - Fast and reproducible quantification of thymosins β4 and β10 in different cell cultures was achieved by ultra high performance liquid chromatography coupled to mass spectrometry. We demonstrated that cancer cell lines all exhibit a higher amount of Tβ10 compared to control cells, whereas the level of Tβ4 is drastically depending on cell lines.

U2 - 10.1039/c0ay00576b

DO - 10.1039/c0ay00576b

M3 - Article

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SN - 1759-9660

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JO - Analytical Methods

JF - Analytical Methods

IS - 3

ER -

UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: A simple, rapid and sensitive quantification method

Abstract

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