@inproceedings{be9d89fad75b415ba6397dd269f01d83,
title = "An all-optical approach to modulate and quantitatively analyse embryo morphogenetic movements by using ultrashort laser pulses",
abstract = "The regulation of morphogenetic movements that shape an embryo during its development remains a challenging issue in developmental biology, and may in certain cases involve mechanical sensitivity. Addressing this issue requires novel experimental approaches. We show that the combination of femtosecond laser pulse-induced ablation and multiphoton microcopy can be used to modulate and quantify morphogenetic movements in Drosophila embryos. We characterized the effects of focused nanoJoule pulse trains in developing embryos. We used targeted ablations to locally modify the embryo structural integrity and modulate morphogenesis. Femtosecond-pulse induced ablation was combined with nonlinear microscopy based on two-photon-excited fluorescence (2PEF) and third-harmonic generation (THG). Correlation-based analysis of microscopy data allowed us to track the outcome of ablations and to analyze tissue deformations. These experiments provided insight into the interplay between gene expression and tissue deformations in developing embryos.",
keywords = "Embryo development, Femtosecond pulse-induced ablation, In vivo tissue imaging, Multiphoton microscopy, Quantitative image sequence analysis",
author = "W. Supatto and D. D{\'e}barre and B. Moulia and E. Farge and E. Beaurepaire",
year = "2006",
month = may,
day = "8",
doi = "10.1117/12.645897",
language = "English",
isbn = "081946130X",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
note = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV ; Conference date: 23-01-2006 Through 25-01-2006",
}