@inproceedings{24089f885cdd49ecae2be9b0bc1ddb39,
title = "Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues",
abstract = "We use a vector field model to analyze the third-harmonic generation (THG) emission patterns for isolated objects illuminated by a Gaussian beam. Simulations and experiments indicate that THG from biological (dielectric) structures is essentially forward-directed, as opposed to e.g. THG from gold particles. We then address the issue of epidetecting forward-emitted light backscattered in a turbid medium. We use Monte Carlo simulations and measurements to analyze the effect of tissue properties (absorption, scattering), and of the geometry of the collecting optics. This analysis provides guidelines for optimizing epidetection in coherent nonlinear microscopy.",
keywords = "Multiphoton microscopy, Third-harmonic generation, Tissue imaging, Turbid media",
author = "Nicolas Olivier and Delphine D{\'e}barre and Emmanuel Beaurepaire",
year = "2008",
month = jun,
day = "18",
doi = "10.1117/12.783376",
language = "English",
isbn = "9780819471895",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Biophotonics",
note = "Biophotonics: Photonic Solutions for Better Health Care ; Conference date: 08-04-2008 Through 10-04-2008",
}