Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain

Aurélie Perier; Christine Gourier; Sylvain Pichard; Julien Husson; Evelyne Lajeunesse; Aurélie Babon; André Menez; Daniel Gillet

doi:10.1016/j.febslet.2007.10.055

Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain

Aurélie Perier
, Christine Gourier
, Sylvain Pichard
, Julien Husson
, Evelyne Lajeunesse
, Aurélie Babon
, André Menez
, Daniel Gillet

CEA/UVSQ/CNRS
Center for Atomic-scale Materials Physics (CAMP)

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

Résumé

We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.

langue originale	Anglais
Pages (de - à)	5480-5484
Nombre de pages	5
journal	FEBS Letters
Volume	581
Numéro de publication	28
Les DOIs	https://doi.org/10.1016/j.febslet.2007.10.055
état	Publié - 27 nov. 2007
Modification externe	Oui

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10.1016/j.febslet.2007.10.055

Contient cette citation

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title = "Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain",

abstract = "We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.",

keywords = "Cell surface engineering, Diphtheria toxin T domain, Flt3-ligand, Membrane anchor",

author = "Aur{\'e}lie Perier and Christine Gourier and Sylvain Pichard and Julien Husson and Evelyne Lajeunesse and Aur{\'e}lie Babon and Andr{\'e} Menez and Daniel Gillet",

year = "2007",

month = nov,

day = "27",

doi = "10.1016/j.febslet.2007.10.055",

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T1 - Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain

AU - Perier, Aurélie

AU - Gourier, Christine

AU - Pichard, Sylvain

AU - Husson, Julien

AU - Lajeunesse, Evelyne

AU - Babon, Aurélie

AU - Menez, André

AU - Gillet, Daniel

PY - 2007/11/27

Y1 - 2007/11/27

N2 - We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.

AB - We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.

KW - Cell surface engineering

KW - Diphtheria toxin T domain

KW - Flt3-ligand

KW - Membrane anchor

U2 - 10.1016/j.febslet.2007.10.055

DO - 10.1016/j.febslet.2007.10.055

M3 - Article

C2 - 17991440

AN - SCOPUS:36148948996

SN - 0014-5793

VL - 581

SP - 5480

EP - 5484

JO - FEBS Letters

JF - FEBS Letters

IS - 28

ER -

Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain

Résumé

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