Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

Marlène Vayssières; Gabrielle Bourgeois; Florian Chardon; Anne Sophie Tillault; Magali Blaud

doi:10.1007/978-1-0716-1386-3_7

Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

Marlène Vayssières
, Gabrielle Bourgeois
, Florian Chardon
, Anne Sophie Tillault
, Magali Blaud

Laboratoire de Probabilités et Modèles Aléatoires
ZMBE Zentrum für Molekularbuiologie der Entzündung
Alberta RNA Research and Training Institute

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › Chapitre › Revue par des pairs

Résumé

Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.

langue originale	Anglais
titre	Methods in Molecular Biology
Editeur	Humana Press Inc.
Pages	65-72
Nombre de pages	8
Les DOIs	https://doi.org/10.1007/978-1-0716-1386-3_7
état	Publié - 1 janv. 2021

Série de publications

Nom	Methods in Molecular Biology
Volume	2300
ISSN (imprimé)	1064-3745
ISSN (Electronique)	1940-6029

Accès au document

10.1007/978-1-0716-1386-3_7

Contient cette citation

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abstract = "Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.",

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Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation. / Vayssières, Marlène; Bourgeois, Gabrielle; Chardon, Florian et al.
Methods in Molecular Biology. Humana Press Inc., 2021. p. 65-72 (Methods in Molecular Biology; Vol 2300).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › Chapitre › Revue par des pairs

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T1 - Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

AU - Vayssières, Marlène

AU - Bourgeois, Gabrielle

AU - Chardon, Florian

AU - Tillault, Anne Sophie

AU - Blaud, Magali

PY - 2021/1/1

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KW - Bridge oligonucleotide

KW - Ligation

KW - Small RNA

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DO - 10.1007/978-1-0716-1386-3_7

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T3 - Methods in Molecular Biology

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PB - Humana Press Inc.

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Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

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