DNA nanostructures made of monomolecular G-Wires

Our interest in the present work is focused on long mono-molecular 1D structures, termed G-Wires. These structures are composed of self-folded long poly(dG) strands where guanines interact through G-Quartets motifs and form superwires with a continuous backbone. These G-Wires are characterized by a narrow length distribution, stiffness, charge polarizability, and a relative resistance to mechanical deformation. They thus present very interesting properties to assemble rigid nanowires, potentially exploitable as structural and functional components, connectors and scaffolds in the context of complex nanodevices assembly. We present here a method we have developed to functionalize these G-Wires in order to adsorb/comb them on various surfaces (Au, Pt) and assemble them into 2D structures using Watson-Crick base pairing. We also show how to easily produce double-stranded DNA molecules with sticky ends of every sequences and lengths. The very simple method described here enables for the first time the production of hetero-or homo-multi-branched structures composed of both double-stranded DNA and four-stranded DNA.