Effect of hydrogenated lecithin on cytotoxicity of liposome

In this study, a hydrogenated lecithin-containing liposome was prepared using a sonicator and microfluidizer at high shear. The surface tension, particle size, turbidity, and transition temperature (T_c) of the liposome were investigated and compared with the commercially available standard surfactant Tween-60. The cytotoxicities were characterized using the MTT method. The surface tension of hydrogenated lecithin was found to be higher than that of Tween-60. The particle size prepared at above T_cwas smaller than that prepared below T_c. The results of the cytotoxicity experiment indicated that Lecinol 10 exhibits the highest IC₅₀value, which shows its high safety in this study.