Individual Control and Quantification of 3D Spheroids in a High-Density Microfluidic Droplet Array

Tomasi et al. describe a microfluidic droplet approach to sequentially modulate culture conditions on 3D spheroids, enabling complex protocols such as co-culture, hydrogel encapsulation, or drug testing. When applied to a drug toxicity experiment on a chip, the platform yields time-resolved, single-cell data that uncover a dynamic response regulated at the spheroid level.