Knockout of UBP34 in Physcomitrella patens reveals the photoaffinity labeling of another closely related IPR protein

Florent Brun; Didier G. Schaefer; Michel Laloue; Martine Gonneau

doi:10.1016/j.plantsci.2004.04.013

Knockout of UBP34 in Physcomitrella patens reveals the photoaffinity labeling of another closely related IPR protein

Florent Brun
, Didier G. Schaefer
, Michel Laloue
, Martine Gonneau

AgroParisTech INRA
University of Lausanne

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

Résumé

UBP34, a soluble 34 kDa protein identified in the moss Physcomitrella patens by photoaffinity labeling with an urea-type cytokinin agonist, belonging to the plant intracellular pathogenesis related (IPR) protein family. This class of proteins is ubiquitous in the plant kingdom but their functions are still unknown. Using a reverse genetic approach we generated knockout mutants at the UBP34 locus by homologous recombination. Knockout plants grown in various conditions do not present any visible phenotypic alteration. However, biochemical and molecular analysis of the knockout transformants reveal the specific photoaffinity labeling of another similar protein.

langue originale	Anglais
Pages (de - à)	471-479
Nombre de pages	9
journal	Plant Science
Volume	167
Numéro de publication	3
Les DOIs	https://doi.org/10.1016/j.plantsci.2004.04.013
état	Publié - 1 sept. 2004
Modification externe	Oui

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10.1016/j.plantsci.2004.04.013

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@article{db92c778463a4bbcb26bc11d402d3501,

title = "Knockout of UBP34 in Physcomitrella patens reveals the photoaffinity labeling of another closely related IPR protein",

abstract = "UBP34, a soluble 34 kDa protein identified in the moss Physcomitrella patens by photoaffinity labeling with an urea-type cytokinin agonist, belonging to the plant intracellular pathogenesis related (IPR) protein family. This class of proteins is ubiquitous in the plant kingdom but their functions are still unknown. Using a reverse genetic approach we generated knockout mutants at the UBP34 locus by homologous recombination. Knockout plants grown in various conditions do not present any visible phenotypic alteration. However, biochemical and molecular analysis of the knockout transformants reveal the specific photoaffinity labeling of another similar protein.",

keywords = "1-(2-chloropyrid-4-yl)-3-phenylurea, Allergen, BA, CPPU, Gene disruption, IP, IPR, Intracellular PR proteins, Physcomitrella patens, benzyladenine, intracellular pathogenesis related, isopentenyladenine",

author = "Florent Brun and Schaefer, \{Didier G.\} and Michel Laloue and Martine Gonneau",

year = "2004",

month = sep,

day = "1",

doi = "10.1016/j.plantsci.2004.04.013",

language = "English",

volume = "167",

pages = "471--479",

journal = "Plant Science",

issn = "0168-9452",

number = "3",

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TY - JOUR

T1 - Knockout of UBP34 in Physcomitrella patens reveals the photoaffinity labeling of another closely related IPR protein

AU - Brun, Florent

AU - Schaefer, Didier G.

AU - Laloue, Michel

AU - Gonneau, Martine

PY - 2004/9/1

Y1 - 2004/9/1

N2 - UBP34, a soluble 34 kDa protein identified in the moss Physcomitrella patens by photoaffinity labeling with an urea-type cytokinin agonist, belonging to the plant intracellular pathogenesis related (IPR) protein family. This class of proteins is ubiquitous in the plant kingdom but their functions are still unknown. Using a reverse genetic approach we generated knockout mutants at the UBP34 locus by homologous recombination. Knockout plants grown in various conditions do not present any visible phenotypic alteration. However, biochemical and molecular analysis of the knockout transformants reveal the specific photoaffinity labeling of another similar protein.

AB - UBP34, a soluble 34 kDa protein identified in the moss Physcomitrella patens by photoaffinity labeling with an urea-type cytokinin agonist, belonging to the plant intracellular pathogenesis related (IPR) protein family. This class of proteins is ubiquitous in the plant kingdom but their functions are still unknown. Using a reverse genetic approach we generated knockout mutants at the UBP34 locus by homologous recombination. Knockout plants grown in various conditions do not present any visible phenotypic alteration. However, biochemical and molecular analysis of the knockout transformants reveal the specific photoaffinity labeling of another similar protein.

KW - 1-(2-chloropyrid-4-yl)-3-phenylurea

KW - Allergen

KW - BA

KW - CPPU

KW - Gene disruption

KW - IP

KW - IPR

KW - Intracellular PR proteins

KW - Physcomitrella patens

KW - benzyladenine

KW - intracellular pathogenesis related

KW - isopentenyladenine

UR - https://www.scopus.com/pages/publications/3142694070

U2 - 10.1016/j.plantsci.2004.04.013

DO - 10.1016/j.plantsci.2004.04.013

M3 - Article

AN - SCOPUS:3142694070

SN - 0168-9452

VL - 167

SP - 471

EP - 479

JO - Plant Science

JF - Plant Science

IS - 3

ER -

Knockout of UBP34 in Physcomitrella patens reveals the photoaffinity labeling of another closely related IPR protein

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