TY - GEN
T1 - Microinstrument for optical monitoring of endothelial cell migration under controlled tension/compression via integrated magnetic composite polymer actuation
AU - Gray, Bonnie L.
AU - Rahbar, Mona
AU - Babataheri, Avin
AU - Barakat, Abdul I.
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/11/26
Y1 - 2014/11/26
N2 - We present a microfabricated platform that allows simultaneous application of controlled stretch/compression forces and fluid flow shear stresses during endothelial cell (EC) live-cell monitoring. Our device employs a highly flexible magnetic composite polymer (M-CP) for actuation of a flexible microchannel system. We combine our M-CP with micropatterned non-magnetic polydimethylsiloxane (PDMS), resulting in flexible microsystems with integrated actuators and microfluidic channels whereby we can optically visualize cells in order to monitor various aspects of cell behavior including migration, proliferation, and morphological changes. The M-CP can be rendered permanently magnetic, so it can be employed for both substrate tension and compression using the same electro-or permanent magnet with pole reversal. We have demonstrated proof-of-concept of an instrument designed to simultaneously stimulate ECs grown in microfluidic channels with both fluid flow and mechanical stretch/compression using the new M-CP actuators.
AB - We present a microfabricated platform that allows simultaneous application of controlled stretch/compression forces and fluid flow shear stresses during endothelial cell (EC) live-cell monitoring. Our device employs a highly flexible magnetic composite polymer (M-CP) for actuation of a flexible microchannel system. We combine our M-CP with micropatterned non-magnetic polydimethylsiloxane (PDMS), resulting in flexible microsystems with integrated actuators and microfluidic channels whereby we can optically visualize cells in order to monitor various aspects of cell behavior including migration, proliferation, and morphological changes. The M-CP can be rendered permanently magnetic, so it can be employed for both substrate tension and compression using the same electro-or permanent magnet with pole reversal. We have demonstrated proof-of-concept of an instrument designed to simultaneously stimulate ECs grown in microfluidic channels with both fluid flow and mechanical stretch/compression using the new M-CP actuators.
U2 - 10.1109/NANO.2014.6968129
DO - 10.1109/NANO.2014.6968129
M3 - Conference contribution
AN - SCOPUS:84919465632
T3 - Proceedings of the IEEE Conference on Nanotechnology
SP - 986
EP - 990
BT - Proceedings of the IEEE Conference on Nanotechnology
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
Y2 - 18 August 2014 through 21 August 2014
ER -