Molecular analysis of microscopic ezrin dynamics by two-photon FRAP

Sylvie Coscoy; François Waharte; Alexis Gautreau; Marianne Martin; Daniel Louvard; Paul Mangeat; Monique Arpin; François Amblard

doi:10.1073/pnas.192084599

Molecular analysis of microscopic ezrin dynamics by two-photon FRAP

Sylvie Coscoy
, François Waharte
, Alexis Gautreau
, Marianne Martin
, Daniel Louvard
, Paul Mangeat
, Monique Arpin
, François Amblard

CNRS
Université de Montpellier

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

Résumé

Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photo- bleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.

langue originale	Anglais
Pages (de - à)	12813-12818
Nombre de pages	6
journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Numéro de publication	20
Les DOIs	https://doi.org/10.1073/pnas.192084599
état	Publié - 1 oct. 2002

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10.1073/pnas.192084599

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abstract = "Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photo- bleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.",

author = "Sylvie Coscoy and Fran{\c c}ois Waharte and Alexis Gautreau and Marianne Martin and Daniel Louvard and Paul Mangeat and Monique Arpin and Fran{\c c}ois Amblard",

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doi = "10.1073/pnas.192084599",

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T1 - Molecular analysis of microscopic ezrin dynamics by two-photon FRAP

AU - Coscoy, Sylvie

AU - Waharte, François

AU - Gautreau, Alexis

AU - Martin, Marianne

AU - Louvard, Daniel

AU - Mangeat, Paul

AU - Arpin, Monique

AU - Amblard, François

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photo- bleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.

AB - Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photo- bleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.

U2 - 10.1073/pnas.192084599

DO - 10.1073/pnas.192084599

M3 - Article

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AN - SCOPUS:0036792051

SN - 0027-8424

VL - 99

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EP - 12818

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 20

ER -

Molecular analysis of microscopic ezrin dynamics by two-photon FRAP

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