Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films

M. C. Clochard; N. Betz; M. Goncalves; C. Bittencourt; J. J. Pireaux; K. Gionnet; G. Déléris; A. L. Le Moël

doi:10.1016/j.nimb.2005.04.029

Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films

M. C. Clochard
, N. Betz
, M. Goncalves
, C. Bittencourt
, J. J. Pireaux
, K. Gionnet
, G. Déléris
, A. L. Le Moël

Institut Pierre Simon Laplace, CNRS and CEA
INSERM U869
UNamur

Résultats de recherche: Contribution à un journal › Article de conférence › Revue par des pairs

Résumé

Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides' activity is maintained.

langue originale	Anglais
Pages (de - à)	208-215
Nombre de pages	8
journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	236
Numéro de publication	1-4
Les DOIs	https://doi.org/10.1016/j.nimb.2005.04.029
état	Publié - 1 juil. 2005
Modification externe	Oui
Evénement	Ionizing Radiation and Polymers Proceedings of the 6th International Symposium on Ionizing Radiation Polymers - Durée: 25 sept. 2004 → 30 sept. 2004

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10.1016/j.nimb.2005.04.029

Contient cette citation

Clochard, M. C., Betz, N., Goncalves, M., Bittencourt, C., Pireaux, J. J., Gionnet, K., Déléris, G., & Le Moël, A. L. (2005). Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 236(1-4), 208-215. https://doi.org/10.1016/j.nimb.2005.04.029

@article{0f94dc68dbd346ae93dc4a5b683b370c,

title = "Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films",

abstract = "Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol\% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides' activity is maintained.",

keywords = "Coupling agent, ELISA, PAA, PVDF, Peptide immobilization, Radiation grafting",

author = "Clochard, \{M. C.\} and N. Betz and M. Goncalves and C. Bittencourt and Pireaux, \{J. J.\} and K. Gionnet and G. D{\'e}l{\'e}ris and \{Le Mo{\"e}l\}, \{A. L.\}",

year = "2005",

month = jul,

day = "1",

doi = "10.1016/j.nimb.2005.04.029",

language = "English",

volume = "236",

pages = "208--215",

journal = "Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms",

issn = "0168-583X",

publisher = "Elsevier B.V.",

number = "1-4",

note = "Ionizing Radiation and Polymers Proceedings of the 6th International Symposium on Ionizing Radiation Polymers ; Conference date: 25-09-2004 Through 30-09-2004",

}

Clochard, MC, Betz, N, Goncalves, M, Bittencourt, C, Pireaux, JJ, Gionnet, K, Déléris, G & Le Moël, AL 2005, 'Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films', Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, VOL. 236, Numéro 1-4, p. 208-215. https://doi.org/10.1016/j.nimb.2005.04.029

Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films. / Clochard, M. C.; Betz, N.; Goncalves, M. et al.
Dans: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol 236, Numéro 1-4, 01.07.2005, p. 208-215.

Résultats de recherche: Contribution à un journal › Article de conférence › Revue par des pairs

TY - JOUR

T1 - Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films

AU - Clochard, M. C.

AU - Betz, N.

AU - Goncalves, M.

AU - Bittencourt, C.

AU - Pireaux, J. J.

AU - Gionnet, K.

AU - Déléris, G.

AU - Le Moël, A. L.

PY - 2005/7/1

Y1 - 2005/7/1

N2 - Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides' activity is maintained.

AB - Introducing hydrophilic functions on poly(vinylidene fluoride) (PVDF) films surface allows the covalent immobilization of peptides. Therefore radiation grafting of acrylic acid (AA) in pre-irradiated PVDF films was achieved to allow surface functionalization with linear and cyclic peptides. Peptides were bound via spacer molecules using EDC as a coupling agent. The reactions were followed by Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode. The amount of immobilized peptides was determined by UV spectroscopy. As well, an uncommon method for PVDF characterization and reactions quantification was used: high-resolution-magic angle spinning nuclear mass spectroscopy (HR-MAS NMR). Spacer saturation of the film surface corresponded to 25 mol% yield meaning that one spacer on 4 carboxylic acids was covalently bound. XPS experiments were also performed to deepen analysis of the surface composition. Peptide density is governed by steric hindrance. ELISA tests showed that the peptides' activity is maintained.

KW - Coupling agent

KW - ELISA

KW - PAA

KW - PVDF

KW - Peptide immobilization

KW - Radiation grafting

U2 - 10.1016/j.nimb.2005.04.029

DO - 10.1016/j.nimb.2005.04.029

M3 - Conference article

AN - SCOPUS:21644468301

SN - 0168-583X

VL - 236

SP - 208

EP - 215

JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

IS - 1-4

T2 - Ionizing Radiation and Polymers Proceedings of the 6th International Symposium on Ionizing Radiation Polymers

Y2 - 25 September 2004 through 30 September 2004

ER -

Peptide immobilization onto radiation grafted PVDF-g-poly(acrylic acid) films

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