Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

K. W. Plaxco; A. M. Mathiowetz; W. A. Goddard

doi:10.1073/pnas.86.24.9841

Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

K. W. Plaxco
, A. M. Mathiowetz
, W. A. Goddard

California Institute of Technology

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

Résumé

Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

langue originale	Anglais
Pages (de - à)	9841-9845
Nombre de pages	5
journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Numéro de publication	24
Les DOIs	https://doi.org/10.1073/pnas.86.24.9841
état	Publié - 1 déc. 1989
Modification externe	Oui

Accès au document

10.1073/pnas.86.24.9841

Contient cette citation

@article{bd6c9baf80e147209a37821ea4efdb6b,

title = "Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA",

abstract = "Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.",

keywords = "Protein-DNA interactions, Salmonella typhimurium, helix-turn-helix motif, molecular modeling, structure-function relationships",

author = "Plaxco, \{K. W.\} and Mathiowetz, \{A. M.\} and Goddard, \{W. A.\}",

year = "1989",

month = dec,

day = "1",

doi = "10.1073/pnas.86.24.9841",

language = "English",

volume = "86",

pages = "9841--9845",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "24",

}

TY - JOUR

T1 - Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

AU - Plaxco, K. W.

AU - Mathiowetz, A. M.

AU - Goddard, W. A.

PY - 1989/12/1

Y1 - 1989/12/1

N2 - Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

AB - Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.

KW - Protein-DNA interactions

KW - Salmonella typhimurium

KW - helix-turn-helix motif

KW - molecular modeling

KW - structure-function relationships

U2 - 10.1073/pnas.86.24.9841

DO - 10.1073/pnas.86.24.9841

M3 - Article

C2 - 2602378

AN - SCOPUS:0024787666

SN - 0027-8424

VL - 86

SP - 9841

EP - 9845

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA

Résumé

Accès au document

Empreinte digitale

Contient cette citation