@inproceedings{546d02f399374bff9ebf9d60eb49a20f,
title = "Probing living cells by terahertz attenuated total reflection: Application to permeabilization dynamics",
abstract = "Using attenuated total reflection (ATR) in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live epithelial cells by saponin, and after electropermeabilization. The origin of the contrast observed between cells and culture medium is addressed by both experimental and theoretical approaches, and gives access to permeabilization dynamics of live cells in real time. We show that terahertz modalities are more sensitive than fluorescence microscopy which is the reference optical technique for electropermeabilization. We propose analytical models for the influx and efflux of non-permeant molecules through permeabilized cell membranes.",
keywords = "ATR, Terahertz, cell membrane permeabilization, detergent, electropermeabilization",
author = "G. Gallot and X. Zheng and M. Grognot and A. Azan and T. Garcia-Sanchez and L. Descamps and Mir, \{L. M.\}",
note = "Publisher Copyright: Copyright {\textcopyright} 2018 SPIE.; Novel Biophotonics Techniques and Applications V 2019 ; Conference date: 26-06-2019 Through 27-06-2019",
year = "2019",
month = jan,
day = "1",
doi = "10.1117/12.2526722",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Arjen Amelink and Nadkarni, \{Seemantini K.\}",
booktitle = "Novel Biophotonics Techniques and Applications V",
}