TY - GEN
T1 - Probing living cells by terahertz attenuated total reflection. Application to permeabilization dynamics
AU - Gallot, G.
AU - Zheng, X.
AU - Grognot, M.
AU - Azan, A.
AU - Garcia-Sanchez, T.
AU - Descamps, L.
AU - Mir, L. M.
N1 - Publisher Copyright:
© 2019 IEEE.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Using attenuated total reflection (ATR) in the terahertz domain [1], we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live epithelial cells. We show that terahertz modalities are more sensitive than fluorescence microscopy which is the reference optical technique for electropermeabilization. We also propose analytical models for the influx and efflux of non-permeant molecules through permeabilized cell membranes by electropermeabilization or by detergent.
AB - Using attenuated total reflection (ATR) in the terahertz domain [1], we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live epithelial cells. We show that terahertz modalities are more sensitive than fluorescence microscopy which is the reference optical technique for electropermeabilization. We also propose analytical models for the influx and efflux of non-permeant molecules through permeabilized cell membranes by electropermeabilization or by detergent.
UR - https://www.scopus.com/pages/publications/85074660342
U2 - 10.1109/CLEOE-EQEC.2019.8871940
DO - 10.1109/CLEOE-EQEC.2019.8871940
M3 - Conference contribution
AN - SCOPUS:85074660342
T3 - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
BT - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
Y2 - 23 June 2019 through 27 June 2019
ER -