TY - GEN
T1 - Probing living cells composition by THz attenuated total reflection - Application to quantitative permeabilization measurement
AU - Grognot, M.
AU - Gallot, G.
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2016/11/28
Y1 - 2016/11/28
N2 - Using Attenuated Total Reflection (ATR) imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live MDCK cells by saponin at low concentration. The origin of the contrast observed between cells and culture medium is addressed by both experimental and theoretical approaches, and demonstrated to give access to permeabilization dynamics. Comparisons with usual permeabilization quantifications methods in biology are given and demonstrate several advantages of our terahertz method.
AB - Using Attenuated Total Reflection (ATR) imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live MDCK cells by saponin at low concentration. The origin of the contrast observed between cells and culture medium is addressed by both experimental and theoretical approaches, and demonstrated to give access to permeabilization dynamics. Comparisons with usual permeabilization quantifications methods in biology are given and demonstrate several advantages of our terahertz method.
UR - https://www.scopus.com/pages/publications/85006110940
U2 - 10.1109/IRMMW-THz.2016.7758371
DO - 10.1109/IRMMW-THz.2016.7758371
M3 - Conference contribution
AN - SCOPUS:85006110940
T3 - International Conference on Infrared, Millimeter, and Terahertz Waves, IRMMW-THz
BT - 41st International Conference on Infrared, Millimeter and Terahertz Waves, IRMMW-THz 2016
PB - IEEE Computer Society
T2 - 41st International Conference on Infrared, Millimeter and Terahertz Waves, IRMMW-THz 2016
Y2 - 25 September 2016 through 30 September 2016
ER -