TY - GEN
T1 - Quantitative measurement of permeabilization in living cells by terahertz attenuated total reflection
AU - Grognot, M.
AU - Gallot, G.
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/11/11
Y1 - 2015/11/11
N2 - Using Attenuated Total Reflection (ATR) imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live MDCK cells by saponin at low concentration. The origin of the contrast observed between cells and culture medium is addressed by both experimental and theoretical approaches, and demonstrated to give access to permeabilization dynamics for two close saponin concentrations (50 and 75 μg/ml).
AB - Using Attenuated Total Reflection (ATR) imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live MDCK cells by saponin at low concentration. The origin of the contrast observed between cells and culture medium is addressed by both experimental and theoretical approaches, and demonstrated to give access to permeabilization dynamics for two close saponin concentrations (50 and 75 μg/ml).
U2 - 10.1109/IRMMW-THz.2015.7327838
DO - 10.1109/IRMMW-THz.2015.7327838
M3 - Conference contribution
AN - SCOPUS:84969180636
T3 - IRMMW-THz 2015 - 40th International Conference on Infrared, Millimeter, and Terahertz Waves
BT - IRMMW-THz 2015 - 40th International Conference on Infrared, Millimeter, and Terahertz Waves
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 40th International Conference on Infrared, Millimeter, and Terahertz Waves, IRMMW-THz 2015
Y2 - 23 August 2015 through 28 August 2015
ER -