Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

Marianne Grognot; Guilhem Gallot

doi:10.1063/1.4930168

Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

Marianne Grognot
, Guilhem Gallot

Institut Polytechnique de Paris

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

Résumé

Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.

langue originale	Anglais
Numéro d'article	103702
journal	Applied Physics Letters
Volume	107
Numéro de publication	10
Les DOIs	https://doi.org/10.1063/1.4930168
état	Publié - 7 sept. 2015

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10.1063/1.4930168

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title = "Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection",

abstract = "Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.",

author = "Marianne Grognot and Guilhem Gallot",

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AU - Gallot, Guilhem

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N2 - Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.

AB - Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.

U2 - 10.1063/1.4930168

DO - 10.1063/1.4930168

M3 - Article

AN - SCOPUS:84941308884

SN - 0003-6951

VL - 107

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 10

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Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

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