TY - GEN
T1 - Removing artifacts in Second Harmonic Generation imaging by interferometry
AU - Pinsard, Maxime
AU - Schmeltz, Margaux
AU - Van Der Kolk, Jarno N.
AU - Patten, Kessen
AU - Ramunno, Lora
AU - Schanne-Klein, Marie Claire
AU - Légaré, François
N1 - Publisher Copyright:
© 2019 IEEE.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - — We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.
AB - — We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.
KW - Interferometry
KW - Multiphoton microscopy
KW - imaging artifacts
UR - https://www.scopus.com/pages/publications/85072763106
U2 - 10.1109/PN.2019.8819578
DO - 10.1109/PN.2019.8819578
M3 - Conference contribution
AN - SCOPUS:85072763106
T3 - 2019 Photonics North, PN 2019
BT - 2019 Photonics North, PN 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2019 Photonics North, PN 2019
Y2 - 21 May 2019 through 23 May 2019
ER -