TY - JOUR
T1 - SiR-XActin
T2 - A Fluorescent Probe for Imaging Actin Dynamics in Live Cells
AU - Nasufovic, Veselin
AU - Kompa, Julian
AU - Lindamood, Halli L.
AU - Blümke, Merle
AU - Dibsy, Rayane
AU - Koch, Birgit
AU - Levario-Diaz, Victoria
AU - Weber, Katharina
AU - Maager, Marlene
AU - Nomerotskaia, Ekaterina
AU - Echard, Arnaud
AU - Cavalcanti-Adam, Elisabetta Ada
AU - Vitriol, Eric A.
AU - Arndt, Hans Dieter
AU - Johnsson, Kai
N1 - Publisher Copyright:
© 2025 The Author(s). Angewandte Chemie International Edition published by Wiley-VCH GmbH.
PY - 2025/12/8
Y1 - 2025/12/8
N2 - Imaging actin-dependent processes in live cells is important for understanding numerous biological processes. However, currently used natural-product-based fluorescent probes for actin filaments affect the dynamics of actin polymerization and can induce undesired cellular phenotypes. Here, we introduce SiR-XActin, a simplified jasplakinolide-based, far-red fluorescent probe that enables bright and photostable staining in various cell types without requiring genetic modifications. Due to its relatively weak binding affinity, the probe exhibits minimal cytotoxicity and labels actin filaments without significantly altering actin dynamics. Furthermore, SiR-XActin is suitable for time-resolved, live-cell super-resolution STED microscopy. Exchanging the SiR fluorophore in SiR-XActin for other fluorophores yields probes in different colors. All these properties make SiR-XActin and its analogs powerful tools for studying actin dynamics using live-cell fluorescence microscopy.
AB - Imaging actin-dependent processes in live cells is important for understanding numerous biological processes. However, currently used natural-product-based fluorescent probes for actin filaments affect the dynamics of actin polymerization and can induce undesired cellular phenotypes. Here, we introduce SiR-XActin, a simplified jasplakinolide-based, far-red fluorescent probe that enables bright and photostable staining in various cell types without requiring genetic modifications. Due to its relatively weak binding affinity, the probe exhibits minimal cytotoxicity and labels actin filaments without significantly altering actin dynamics. Furthermore, SiR-XActin is suitable for time-resolved, live-cell super-resolution STED microscopy. Exchanging the SiR fluorophore in SiR-XActin for other fluorophores yields probes in different colors. All these properties make SiR-XActin and its analogs powerful tools for studying actin dynamics using live-cell fluorescence microscopy.
KW - F-actin
KW - Fluorescent probes
KW - Jasplakinolide derivatives
KW - No-wash live-cell imaging
KW - STED microscopy
UR - https://www.scopus.com/pages/publications/105019221715
U2 - 10.1002/anie.202509285
DO - 10.1002/anie.202509285
M3 - Article
AN - SCOPUS:105019221715
SN - 1433-7851
VL - 64
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 50
M1 - e202509285
ER -